• Obtained his B.S degree in Chemistry at Birzeit University in Palestine (1996), M.S degree in Bioinorganic Chemistry at Bergen University in Norway (1998; advisor: Dr. Einar Sletten), and a Ph.D degree in Bioinorganic Chemistry at Emory University in Atlanta (2002; advisor: Dr. Luigi Marzilli). His M.S. and Ph.D studies focused on studying the interactions of cisplatin, a leading anticancer drug, with DNA by using Nuclear Magnetic Resonance (NMR) and other biophysical methods. Dr. Saad's postdoctoral work in Dr. Michael Summers' lab (HHMI, UMBC) focused on the mechanism by which retroviruses are directed to specific cellular membranes for assembly. Dr. Saad joined the UAB faculty in 2007.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2019 The tuberculosis necrotizing toxin is an NAD and NADP glycohydrolase with distinct enzymatic propertiesJournal of Biological Chemistry.  294:3024-3036. 2019
    2018 Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assemblyJournal of Biological Chemistry.  293:18828-18840. 2018
    2018 The matrix domain of the Gag protein from avian sarcoma virus contains a PI(4,5)P 2 -binding site that targets Gag to the cell peripheryJournal of Biological Chemistry.  293:18841-18853. 2018
    2017 Position of O-acetylation within the capsular repeat unit impacts the biological properties of pneumococcal serotypes 33A and 33FInfection and Immunity.  85. 2017
    2017 Discovery of novel pneumococcal serotype 35D, a natural WciG-deficient variant of serotype 35BJournal of Clinical Microbiology.  55:1416-1425. 2017
    2017 The interplay between calmodulin and membrane interactions with the pleckstrin homology domain of aktJournal of Biological Chemistry.  292:251-263. 2017
    2016 Pneumococcus with the "6E" CPS locus produces serotype 6B capsular polysaccharideJournal of Clinical Microbiology.  54:967-971. 2016
    2015 Structural and biophysical characterization of the interactions between calmodulin and the pleckstrin homology domain of AktJournal of Biological Chemistry.  290:27403-27413. 2015
    2015 Erratum: Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units (The Journal of Biological Chemistry (2013) 288 (25976-25985))Journal of Biological Chemistry.  290:26474-26475. 2015
    2015 Targeting the Early Step of Building Block Organization in Viral Capsid AssemblyACS Chemical Biology.  10:1785-1790. 2015
    2015 Editorial: Role of lipids in virus assemblyFrontiers in Microbiology.  6. 2015
    2015 Structural and molecular determinants of HIV-1 Gag binding to the plasma membraneFrontiers in Microbiology.  6. 2015
    2014 Solution structure of calmodulin bound to the binding domain of the HIV-1 matrix proteinJournal of Biological Chemistry.  289:8697-8705. 2014
    2014 Spectrum of pneumococcal serotype 11A variants results from incomplete loss of capsule O-acetylationJournal of Clinical Microbiology.  52:758-765. 2014
    2013 Discovery of streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating unitsJournal of Biological Chemistry.  288:25976-25985. 2013
    2013 Structural and biophysical characterization of the interactions between the death domain of fas receptor and calmodulinJournal of Biological Chemistry.  288:21898-21908. 2013
    2012 Structural characterization of Streptococcus pneumoniae serotype 9A capsule polysaccharide reveals role of glycosyl 6-O-acetyltransferase wcjE in serotype 9V capsule biosynthesis and immunogenicityJournal of Biological Chemistry.  287:13996-14003. 2012
    2012 Role of the HIV-1 matrix protein in Gag intracellular trafficking and targeting to the plasma membrane for virus assemblyFrontiers in Microbiology.  3. 2012
    2011 NMR, biophysical, and biochemical studies reveal the minimal calmodulin binding domain of the HIV-1 matrix proteinJournal of Biological Chemistry.  286:33533-33543. 2011
    2011 Single-stranded oligonucleotide adducts formed by Pt complexes favoring left-handed base canting: Steric effect of flanking residues and relevance to DNA adducts formed by Pt anticancer drugsInorganic Chemistry.  50:8608-8620. 2011
    2011 NMR studies of models having the Pt(d(GpG)) 17-membered macrocyclic ring formed in DNA by platinum anticancer drugs: Pt complexes with bulky chiral diamine ligandsInorganic Chemistry.  50:4559-4571. 2011
    2010 Binding of calmodulin to the HIV-1 matrix protein triggers myristate exposureJournal of Biological Chemistry.  285:41911-41920. 2010
    2010 Myristate exposure in the human immunodeficiency virus type 1 matrix protein is modulated by pHBiochemistry.  49:9551-9562. 2010
    2010 Basic coordination chemistry relevant to DNA adducts formed by the cisplatin anticancer drug. NMR studies on compounds with sterically crowded chiral ligandsInorganic Chemistry.  49:5573-5583. 2010
    2003 Chiral discrimination in the formation reaction and at equilibrium for N,N,N′9N′-tetramethyl-1,2-diaminocyclohexane-PtG2 complexesDalton Transactions.  872-879. 2003
    2002 Factors influencing conformer equilibria in retro models of cisplatin-DNA adducts as revealed by moderately dynamic (N,N′-dimethyl-2,3-diaminobutane)PtG2 retro models (G = a guanine derivative)Inorganic Chemistry.  41:4923-4935. 2002
    2002 Modification of second-sphere communication, leading to an unusually high abundance of the head-to-head conformer of cisplatin cross-link retro modelsInorganic Chemistry.  41:546-557. 2002

    Research Overview

  • HIV-Host Interactions and Fas-Mediated Apoptosis - My lab is interested in understanding biological mechanisms in virology and cancer at the atomic level. Our biggest state-of-the-art toy in the lab is called Nuclear Magnetic Resonance (NMR). We apply this technology to assess how proteins interact with membranes, other proteins, small molecules, and sugars. In collaboration with UAB colleagues, we are starting to apply NMR Spectroscopy methods to detect and quantify metabolites in animal cells and tissues. Our main focus in the virology field is aimed at elucidating molecular mechanisms of HIV assembly. Our cancer projects include those aimed at characterization of protein-protein interactions pertaining to Fas-mediated apoptosis in cholangiocarcinoma. Most recently, we have been investigating at the structural level how the Akt protein interacts with lipids and proteins to facilitate translocation to the plasma membrane for activation in many cancer cells.

    Keywords - HIV, NMR, Cancer, Drug design
  • Education And Training

  • Doctor of Philosophy in Chemistry, Emory University 2002
  • Master's Level Degree in Chemistry, Bergen University College 1998
  • Bachelor's Level Degree in Chemistry, Birzeit University 1996
  • Full Name

  • Jamil Saad
  • Fax

  • 205-996-4008