Positions

Overview

  • Obtained his B.S degree in Chemistry at Birzeit University in Palestine (1996), M.S degree in Bioinorganic Chemistry at Bergen University in Norway (1998; advisor: Dr. Einar Sletten), and a Ph.D degree in Bioinorganic Chemistry at Emory University in Atlanta (2002; advisor: Dr. Luigi Marzilli). His M.S. and Ph.D studies focused on studying the interactions of cisplatin, a leading anticancer drug, with DNA by using Nuclear Magnetic Resonance (NMR) and other biophysical methods. Dr. Saad's postdoctoral work in Dr. Michael Summers' lab (HHMI, UMBC) focused on the mechanism by which retroviruses are directed to specific cellular membranes for assembly. Dr. Saad joined the UAB faculty in 2007.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2019 Structural and biophysical characterizations of HIV-1 matrix trimer binding to lipid nanodiscs shed light on virus assembly.Journal of Biological Chemistry2019
    2019 The KN-93 Molecule Inhibits Calcium/Calmodulin-Dependent Protein Kinase II (CaMKII) Activity by Binding to Ca 2+ /CaMJournal of Molecular Biology.  431:1440-1459. 2019
    2019 The tuberculosis necrotizing toxin is an NAD and NADP glycohydrolase with distinct enzymatic propertiesJournal of Biological Chemistry.  294:3024-3036. 2019
    2018 Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assemblyJournal of Biological Chemistry.  293:18828-18840. 2018
    2018 The matrix domain of the Gag protein from avian sarcoma virus contains a PI(4,5)P 2 -binding site that targets Gag to the cell peripheryJournal of Biological Chemistry.  293:18841-18853. 2018
    2017 Solution Structure and Membrane Interaction of the Cytoplasmic Tail of HIV-1 gp41 ProteinStructure.  25:1708-1718.e5. 2017
    2017 Position of O-acetylation within the capsular repeat unit impacts the biological properties of pneumococcal serotypes 33A and 33FInfection and Immunity.  85. 2017
    2017 Derepression of SaPIbov1 Is Independent of φNM1 Type 2 dUTPase Activity and Is Inhibited by dUTP and dUMPJournal of Molecular Biology.  429:1570-1580. 2017
    2017 Discovery of novel pneumococcal serotype 35D, a natural WciG-deficient variant of serotype 35BJournal of Clinical Microbiology.  55:1416-1425. 2017
    2017 The interplay between calmodulin and membrane interactions with the pleckstrin homology domain of aktJournal of Biological Chemistry.  292:251-263. 2017
    2016 Pneumococcus with the "6E" CPS locus produces serotype 6B capsular polysaccharideJournal of Clinical Microbiology.  54:967-971. 2016
    2016 Identification of the calmodulin-binding domains of Fas death receptorPLoS ONE.  11. 2016
    2015 Structural and biophysical characterization of the interactions between calmodulin and the pleckstrin homology domain of AktJournal of Biological Chemistry.  290:27403-27413. 2015
    2015 Erratum: Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units (The Journal of Biological Chemistry (2013) 288 (25976-25985))Journal of Biological Chemistry.  290:26474-26475. 2015
    2015 Targeting the Early Step of Building Block Organization in Viral Capsid AssemblyPediatric Annals.  10:1785-1790. 2015
    2015 Editorial: Role of lipids in virus assemblyFrontiers in Microbiology.  6. 2015
    2015 Structural and molecular determinants of HIV-1 Gag binding to the plasma membraneFrontiers in Microbiology.  6. 2015
    2014 Solution structure of calmodulin bound to the binding domain of the HIV-1 matrix proteinJournal of Biological Chemistry.  289:8697-8705. 2014
    2014 HIV: A vicTIMTrends in Microbiology.  22:603-604. 2014
    2014 Spectrum of pneumococcal serotype 11A variants results from incomplete loss of capsule O-acetylationJournal of Clinical Microbiology.  52:758-765. 2014
    2013 Discovery of streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating unitsJournal of Biological Chemistry.  288:25976-25985. 2013
    2013 Structural and biophysical characterization of the interactions between the death domain of fas receptor and calmodulinJournal of Biological Chemistry.  288:21898-21908. 2013
    2013 Trio engagement via plasma membrane phospholipids and the myristoyl moiety governs HIV-1 matrix binding to bilayersProceedings of the National Academy of Sciences.  110:3525-3530. 2013
    2012 Structural characterization of Streptococcus pneumoniae serotype 9A capsule polysaccharide reveals role of glycosyl 6-O-acetyltransferase wcjE in serotype 9V capsule biosynthesis and immunogenicityJournal of Biological Chemistry.  287:13996-14003. 2012
    2012 Role of the HIV-1 matrix protein in Gag intracellular trafficking and targeting to the plasma membrane for virus assemblyFrontiers in Microbiology.  3. 2012
    2011 The staphylococcus aureus pathogenicity island 1 protein gp6 functions as an internal scaffold during capsid size determinationJournal of Molecular Biology.  412:710-722. 2011
    2011 NMR, biophysical, and biochemical studies reveal the minimal calmodulin binding domain of the HIV-1 matrix proteinJournal of Biological Chemistry.  286:33533-33543. 2011
    2011 Single-stranded oligonucleotide adducts formed by Pt complexes favoring left-handed base canting: Steric effect of flanking residues and relevance to DNA adducts formed by Pt anticancer drugsInorganic Chemistry.  50:8608-8620. 2011
    2011 NMR studies of models having the Pt(d(GpG)) 17-membered macrocyclic ring formed in DNA by platinum anticancer drugs: Pt complexes with bulky chiral diamine ligandsInorganic Chemistry.  50:4559-4571. 2011
    2010 Binding of calmodulin to the HIV-1 matrix protein triggers myristate exposureJournal of Biological Chemistry.  285:41911-41920. 2010
    2010 Myristate exposure in the human immunodeficiency virus type 1 matrix protein is modulated by pHBiomarker Insights.  49:9551-9562. 2010
    2010 Basic coordination chemistry relevant to DNA adducts formed by the cisplatin anticancer drug. NMR studies on compounds with sterically crowded chiral ligandsInorganic Chemistry.  49:5573-5583. 2010
    2010 Reverse Micelle Encapsulation of Membrane-Anchored Proteins for Solution NMR StudiesStructure.  18:9-16. 2010
    2010 Targeting of murine leukemia virus gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the matrixJournal of Virology.  84:503-515. 2010
    2009 Targeting of MuLV gag to the plasma membrane is mediated by PI(4,5)P2 and phosphatidylserineRetrovirology.  6. 2009
    2009 Origins of the distortions in the base pair step adjacent to platinum anticancer drug-DNA adducts. Fundamental NMR solution studies utilizing right-handed cross-link models having 5′- and 3′-flanking residuesJournal of the American Chemical Society.  131:12314-12324. 2009
    2008 Structure of the Myristylated Human Immunodeficiency Virus Type 2 Matrix Protein and the Role of Phosphatidylinositol-(4,5)-Bisphosphate in Membrane TargetingJournal of Molecular Biology.  382:434-447. 2008
    2007 Mutations that mimic phosphorylation of the HIV-1 matrix protein do not perturb the myristyl switchProtein Science.  16:1793-1797. 2007
    2007 Point Mutations in the HIV-1 Matrix Protein Turn Off the Myristyl SwitchJournal of Molecular Biology.  366:574-585. 2007
    2006 Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assemblyProceedings of the National Academy of Sciences.  103:11364-11369. 2006
    2003 Chiral discrimination in the formation reaction and at equilibrium for N,N,N′9N′-tetramethyl-1,2-diaminocyclohexane-PtG2 complexesSocial Psychiatry and Psychiatric Epidemiology.  872-879. 2003
    2002 Factors influencing conformer equilibria in retro models of cisplatin-DNA adducts as revealed by moderately dynamic (N,N′-dimethyl-2,3-diaminobutane)PtG2 retro models (G = a guanine derivative)Inorganic Chemistry.  41:4923-4935. 2002
    2002 Dramatic 5′-residue effect on conformer distribution of short oligonucleotide retro models of the cisplatin-DNA cross-link: Implications for the lippard and cross-link distorted base pair steps present in cisplatin-DNA duplex adductsJournal of the American Chemical Society.  124:1558-1559. 2002
    2002 Modification of second-sphere communication, leading to an unusually high abundance of the head-to-head conformer of cisplatin cross-link retro modelsInorganic Chemistry.  41:546-557. 2002
    2001 Relationship of solution and protein-bound structures of DNA duplexes with the major intrastrand cross-link lesions formed on cisplatin binding to DNAJournal of the American Chemical Society.  123:2764-2770. 2001

    Research Overview

  • HIV-Host Interactions and Fas-Mediated Apoptosis - My lab is interested in understanding biological mechanisms in virology and cancer at the atomic level. Our biggest state-of-the-art toy in the lab is called Nuclear Magnetic Resonance (NMR). We apply this technology to assess how proteins interact with membranes, other proteins, small molecules, and sugars. In collaboration with UAB colleagues, we are starting to apply NMR Spectroscopy methods to detect and quantify metabolites in animal cells and tissues. Our main focus in the virology field is aimed at elucidating molecular mechanisms of HIV assembly. Our cancer projects include those aimed at characterization of protein-protein interactions pertaining to Fas-mediated apoptosis in cholangiocarcinoma. Most recently, we have been investigating at the structural level how the Akt protein interacts with lipids and proteins to facilitate translocation to the plasma membrane for activation in many cancer cells.

    Keywords - HIV, NMR, Cancer, Drug design
  • Education And Training

  • Doctor of Philosophy in Chemistry, Emory University 2002
  • Master's Level Degree in Chemistry, Bergen University College 1998
  • Bachelor's Level Degree in Chemistry, Birzeit University 1996
  • Full Name

  • Jamil Saad
  • Fax

  • 205-996-4008