• Dr. Richard A. Dluhy is currently Professor of Chemistry and Chair of the Department of Chemistry at the University of Alabama at Birmingham. He received his B.S. degree in Biochemistry from the University of Connecticut, and his Ph.D. in Physical Chemistry from Rutgers University. He was an NRC Post-Doctoral Research Associate at the National Research Council of Canada in Ottawa, Canada. Prior to his academic career, Dr. Dluhy worked in the pharmaceutical industry at Hoffman-LaRoche (Nutley, NJ), and in the Biotechnology and Chemical Methods Development groups at Battelle Memorial Institute (Columbus, Ohio). Prior to arriving at UAB, he was Associate Professor and Professor in the Department of Chemistry at the University of Georgia (Athens), where he was also Associate Department Head and Director of the Nanoscale Science and Research Center. His research interests span a range of topics related to biomedical spectroscopy, and surface and interfacial analysis, particularly the development and application of infrared and Raman spectroscopy to the study the biomolecular assemblies, and chemically assembled nanomaterials at surfaces. Dr. Dluhy’s research has been supported by the National Institutes of Health, National Science Foundation, Howard Hughes Medical Institute, US Department of Energy and the European Union. His work has been internationally recognized through numerous invited lectures; in addition, he was appointed Professeur Visité, Centre de Physique Moléculaire Optique et Hertzienne at the Université Bordeaux, Bordeaux, France, was awarded an Alexander von Humboldt Research Award from the Humboldt Foundation in Bonn, Germany, and was a DAAD Fellow at the Robert Koch Institute, Berlin, Germany.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2018 Non-invasive analysis of stored red blood cells using diffuse resonance Raman spectroscopy 2018
    2018 A rapid and simple chemical method for the preparation of Ag colloids for surface-enhanced Raman spectroscopy using the Ag mirror reactionVibrational Spectroscopy.  98:1-7. 2018
    2018 Characterization of storage-induced red blood cell hemolysis using Raman spectroscopyLaboratory Medicine.  49:298-310. 2018
    2017 Variable Selection and Biomarker Correlation in the Analysis of Mycoplasma pneumoniae Strains by Surface-Enhanced Raman SpectroscopyAnalytical Letters.  50:2412-2425. 2017
    2016 Label-Free Detection of Glycan–Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS) 2016
    2015 Detection of neuraminidase stalk motifs associated with enhanced N1 subtype influenza A virulence via Raman spectroscopy 2015
    2015 Specificity and strain-typing capabilities of nanorod array-surface enhanced raman spectroscopy for Mycoplasma pneumoniae detectionPLoS ONE.  10. 2015
    2014 The multivariate detection limit for Mycoplasma pneumoniae as determined by nanorod array-surface enhanced Raman spectroscopy and comparison with limit of detection by qPCR 2014
    2014 Layer-by-layer polyelectrolyte encapsulation of mycoplasma pneumoniae for enhanced raman detection 2014
    2014 Identification of virulence determinants in influenza virusesAnalytical Chemistry.  86:6911-6917. 2014
    2013 Identification of mycobacteria based on spectroscopic analyses of mycolic acid profiles 2013
    2013 Ag nanorod based surface-enhanced Raman spectroscopy applied to bioanalytical sensingJournal of Biophotonics.  6:20-35. 2013
    2013 Detection of genetic markers related to high pathogenicity in influenza by SERS 2013
    2012 Direct optical detection of viral nucleoprotein binding to an anti-influenza aptamerAnalytical Chemistry.  84:5501-5508. 2012
    2012 Detection and differentiation of avian mycoplasmas by surface-enhanced raman spectroscopy based on a silver nanorod arrayApplied and Environmental Microbiology.  78:1930-1935. 2012
    2011 Erratum: The use of aligned silver nanorod arrays prepared by oblique angle deposition as surface-enhanced raman scattering substrates (The Journal of Physical Chemistry C (2008) 112 (895-901) DOI:10.1021/jp075288u) 2011
    2011 Development of silver nanorod array based fiber optic probes for SERS detection 2011
    2011 Detection of viral nucleoprotein binding to anti-influenza aptamers via SERSChemical Communications.  47:8635-8637. 2011
    2011 Removal of surface contamination and self-assembled monolayers (SAMs) from silver (Ag) nanorod substrates by plasma cleaning with argonApplied Spectroscopy.  65:66-74. 2011
    2010 SERS-based viral fingerprinting: Current capabilities and challengesProceedings of SPIE.  7703. 2010
    2010 Detection of Mycoplasma pneumoniaein simulated and true clinical throat swab specimens by nanorod array-surface-enhanced raman spectroscopyPLoS ONE.  5. 2010
    2010 The SERS response of semiordered Ag nanorod arrays fabricated by template oblique angle depositionJournal of Raman Spectroscopy.  41:1112-1118. 2010
    2010 Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced raman spectroscopyPLoS ONE.  5. 2010
    2010 Identification of individual genotypes of measles virus using surface enhanced Raman spectroscopy 2010
    2009 Novel nanoarray SERS substrates used for high sensitivity virus biosensing and classificationACS Symposium Series.  1016:99-114. 2009
    2009 Quantitative surface-enhanced Raman spectroscopy based analysis of microRNA mixturesApplied Spectroscopy.  63:1107-1114. 2009
    2009 Optimization of a multi-well array SERS chiProceedings of SPIE.  7321. 2009
    2009 Fabrication and characterization of a multiwell array SERS chip with biological applicationsBiosensors and Bioelectronics.  24:3663-3670. 2009
    2009 Surface enhanced Raman scattering from an ag nanorod array substrate: The site dependent enhancement and layer absorbance effect 2009
    2009 The role of the nanospine in the nanocomb arrays for surface enhanced Raman scatteringApplied Physics Letters.  94. 2009
    2008 Rapid microRNA (miRNA) detection and classification via surface-enhanced Raman spectroscopy (SERS)Biosensors and Bioelectronics.  24:917-922. 2008
    2008 Infectious agent detection with SERS-active silver nanorod arrays prepared by oblique angle depositionIEEE Sensors Journal.  8:863-870. 2008
    2008 Novel nanostructures for SERS biosensing 2008
    2008 Identification and classification of respiratory syncytial virus (RSV) strains by surface-enhanced Raman spectroscopy and multivariate statistical techniques 2008
    2008 The use of aligned silver nanorod arrays prepared by oblique angle deposition as surface enhanced raman scattering substrates 2008
    2007 Structure and conformation of the disulfide bond in dimeric lung surfactant peptides SP-B1-25 and SP-B8-25 2007
    2006 Silver nanorod arrays as high sensitive SERS substrates for viral detectionProceedings of SPIE.  6324. 2006
    2006 Angle dependent surface enhanced Raman scattering obtained from a Ag nanorod array substrateApplied Physics Letters.  89. 2006
    2006 Two-dimensional infrared correlation spectroscopy: Global phase angles for specific analytical systemsJournal of Molecular Structure.  799:48-51. 2006
    2006 Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate 2006
    2006 The application of two-dimensional correlation spectroscopy to surface and interfacial analysisSurface and Interface Analysis.  38:1481-1496. 2006
    2006 Micelle density regulated by a reversible switch of protein secondary structureJournal of the American Chemical Society.  128:12014-12019. 2006
    2006 Aligned silver nanorod arrays as substrates for surface-enhanced infrared absorption spectroscopyApplied Spectroscopy.  60:906-913. 2006
    2006 Designing nanostructures for sensor applicationsJournal of Electronic Materials.  35:846-851. 2006
    2006 Hemoglobin is expressed by alveolar epithelial cellsJournal of Biological Chemistry.  281:5668-5676. 2006
    2006 Polarized surface enhanced Raman and absorbance spectra of aligned silver nanorod arrays 2006
    2006 Membrane-mimetic films of asymmetric phosphatidylcholine lipid bolaamphiphiles 2006
    2006 A general model-based approach to two-dimensional infrared correlation spectroscopy incorporating the global phase angleApplied Spectroscopy.  60:1279-1284. 2006
    2005 Analysis of photoinitiated polymerization in a membrane mimetic film using infrared spectroscopy and near-IR Raman microscopyColloids and Surfaces B: Biointerfaces.  46:226-232. 2005
    2005 Aligned silver nanorod arrays produce high sensitivity surface-enhanced Raman spectroscopy substratesApplied Physics Letters.  87. 2005
    2005 Characterization of the low-energy electronic excited states of benzoyl-substituted ruthenocenes 2005
    2005 Structure and properties of phospholipid-peptide monolayers containing monomeric SP-B 1-25: I. Phases and morphology by epifluorescence microscopyBiophysical Chemistry.  113:223-232. 2005
    2005 Structure and properties of phospholipid-peptide monolayers containing monomeric SP-B 1-25: II. Peptide conformation by infrared spectroscopyBiophysical Chemistry.  113:233-244. 2005
    2004 2D IR analyses of rate processes in lipid - Antibiotic monomolecular filmsVibrational Spectroscopy.  36:167-177. 2004
    2004 Vibrational characterization of a planar-supported model bilayer system utilizing surface-enhanced Raman scattering (SERS) and infrared reflection-absorption spectroscopy (IRRAS)Colloids and Surfaces A: Physicochemical and Engineering Aspects.  243:157-167. 2004
    2004 Kv correlation analysis. A quantitative two-dimensional IR correlation method for analysis of rate processes with exponential functions 2004
    2003 Deacylated pulmonary surfactant protein SP-C transforms from α-helical to amyloid fibril structure via a pH-dependent mechanism: An infrared structural investigationBiophysical Journal.  85:2417-2429. 2003
    2003 Thermally induced micro-domain formation in fatty acid Langmuir-Blodgett films observed by attenuated total reflectance infrared spectroscopyColloids and Surfaces A: Physicochemical and Engineering Aspects.  219:125-145. 2003
    2003 Effect of hydrophobic surfactant proteins SP-B and SP-C on binary phospholipid monolayers II. Infrared external reflectance-absorption spectroscopyBiophysical Journal.  84:326-340. 2003
    2002 Classical metallocenes as photoinitiators for the anionic polymerization of an alkyl 2-cyanoacrylateMacromolecules.  35:9648-9652. 2002
    2002 Effect of hydrophobic surfactant protein SP-C on binary phospholipid monolayers. Molecular machinery at the air/water interfaceBiophysical Chemistry.  99:209-228. 2002
    2002 Effect of hydrophobic surfactant proteins SP-B and SP-C on phospholipid monolayers. Protein structure studied using 2D IR and βv correlation analysisBiophysical Journal.  83:2126-2141. 2002
    2002 Development of a novel dual-layer thick Ag substrate for surface-enhanced Raman scattering (SERS) of self-assembled monolayers 2002
    2002 A study of binary phospholipid mixtures at the air-water interface using infrared reflection-absorption spectroscopy and 2D IR βv correlation analysis 2002
    2002 A membrane-mimetic barrier for cell encapsulation 2002
    2001 βν-correlation analysis: A modified two-dimensional infrared correlation method for determining relative rates of intensity change 2001
    2000 Application of 2D IR correlation analysis to phase transitions in Langmuir monolayer filmsColloids and Surfaces A: Physicochemical and Engineering Aspects.  171:225-239. 2000
    2000 Cytomimetic biomaterials. 4. In-situ photopolymerization of phospholipids on an alkylated surfaceMacromolecules.  33:4205-4212. 2000
    2000 Novel fiber-optic interface for unenhanced external reflection Raman spectroscopy of supported monolayers 2000
    2000 Infrared spectroscopy of biophysical monomolecular films at interfaces: Theory and applicationsApplied Spectroscopy Reviews.  35:315-351. 2000
    2000 Pressure-dependent changes in the infrared C-H vibrations of monolayer films at the air/water interface revealed by two-dimensional infrared correlation spectroscopyApplied Spectroscopy.  54:956-962. 2000
    1999 Reflection-absorption FT-IR spectroscopy of vinyl octadecanoate at the air/water interfaceColloids and Surfaces A: Physicochemical and Engineering Aspects.  156:215-222. 1999
    1999 Reflection-absorption FT-IR spectroscopy of pentadecanoic acid at the air/water interfaceColloids and Surfaces A: Physicochemical and Engineering Aspects.  146:49-61. 1999
    1999 Effect of hydrophobic surfactant peptides SP-B and SP-C on binary phospholipid monolayers. I. Fluorescence and dark-field microscopyBiophysical Journal.  77:903-914. 1999
    1999 Structures of yeast vesicle trafficking proteinsProtein Science.  8:2465-2473. 1999
    1998 Arrondo, JLR 96, 53Chemistry and Physics of Lipids.  96:159. 1998
    1998 FTIR external reflectance studies of lipid monolayers at the air-water interface: Applications to pulmonary surfactantAIP Conference Proceedings.  430:320-323. 1998
    1998 Infrared spectroscopy of aqueous biophysical monolayersThin Solid Films.  327-329:308-314. 1998
    1998 On the use of deuterated phospholipids for infrared spectroscopic studies of monomolecular films: A thermodynamic analysis of single and binary component phospholipid monolayersChemistry and Physics of Lipids.  96:81-97. 1998
    1998 Use of polarized infrared external reflection spectroscopy to study phospholipid monolayers at the air-water interfaceAIP Conference Proceedings.  430:641-644. 1998
    1997 Preparation of atomically smooth germanium substrates for infrared spectroscopic and scanning probe microscopic characterization of organic monolayers 1997
    1997 Silver nanorod arrays can distinguish virus strainsPhys Rev Lett.  78:1667-1670. 1997
    1997 Spectroscopy & microscopy of aqueous lipid monolayers with applications to pulmonary surfactant: IR and fluorescence studies.Biophysical Journal.  72:MP271-MP271. 1997
    1996 In-situ and ex-situ structural analysis of phospholipid-supported planar bilayers using infrared spectroscopy and atomic force microscopyThin Solid Films.  284-285:381-386. 1996
    1996 Books: Spectroscopy on the SurfaceAnalytical Chemistry.  68:428A-428A. 1996
    1995 An investigation of miscibility in monolayer films of phosphocholine-phosphoglycerol binary mixturesColloids and Surfaces A: Physicochemical and Engineering Aspects.  102:231-245. 1995
    1995 Use of Internal Reflectance Infrared Spectroscopy for thein SituStudy of Supported Lipid MonolayersApplied Spectroscopy.  49:526-531. 1995
    1995 Vibrational spectroscopy of biophysical monolayers. Applications of IR and Raman spectroscopy to biomembrane model systems at interfacesSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.  51:1413-1447. 1995
    1994 A combined IR and 31P NMR method for determining fractional composition in binary phospholipid monolayer films. Applications to phosphocholine: Phosphoglycerol mixturesProceedings of SPIE.  2089:514-515. 1994
    1994 Microdomain size determination in Langmuir-Blodgett films of fatty acid binary mixturesProceedings of SPIE.  2089:168-169. 1994
    1994 Preparation of atomically smooth Ge substrates for combined IR spectroscopy and scanning probe microscopy of organic monolayersProceedings of SPIE.  2089:486-487. 1994
    1994 Structural characterization and nanometer-scale domain formation in phospholipid model membranes by infrared spectroscopy and scanning tunneling microscopyProceedings of SPIE.  2089:350-351. 1994
    1994 Metastability in monolayer films transferred onto solid substrates by the Langmuir-Blodgett method: IR evidence for transfer-induced phase transitionsApplied Spectroscopy.  48:1196-1203. 1994
    1994 Structural characterization and nanometer-scale domain formation in a model phospholipid bilayer as determined by infrared spectroscopy and scanning tunneling microscopyThe Journal of physical chemistry.  98:1010-1021. 1994
    1993 Combined infrared and 31P NMR spectroscopic method for determining the fractional composition in langmuir-blodgett films of binary phospholipid mixturesApplied Spectroscopy.  47:1015-1023. 1993
    1993 Kinetic and Spectroscopic Studies of Hydrophilic Amino Acid Substitutions in the Hydrophobic Pocket of Human Carbonic Anhydrase IIBiochemistry.  32:4496-4505. 1993
    1993 Surface chemistry of binary mixtures of phospholipids in monolayers. Infrared studies of surface composition at varying surface pressures in a pulmonary surfactant model system.Biochemistry.  32:3169-3177. 1993
    1993 Identification of Phosphocholine Plasmalogen as a Lipid Component in Mammalian Pulmonary Surfactant Using High-Resolution 31P NMR SpectroscopyBiochemistry.  32:27-31. 1993
    1989 Direct determination of conformational disorder in biological membranes by ft-ir spectroscopyProceedings of SPIE.  1145:598-599. 1989
    1989 Langmuir revisited: Using infrared reflectance spectroscopy to determine monolayer structure at liquid interfacesProceedings of SPIE.  1145:22-27. 1989
    1989 The interfacial structure of phospholipid monolayer films: an infrared reflectance studyJournal of Molecular Structure.  214:93-109. 1989
    1989 Understanding the interfacial structure of aqueous phospholipid monolayer films via external reflection FT-IR spectroscopyProceedings of SPIE.  1145:255-256. 1989
    1989 Conformations and Orientations of a Signal Peptide Interacting with Phospholipid MonolayersBiochemistry.  28:2789-2797. 1989
    1989 Conformations and Orientations of a Signal Peptide Interacting with Phospholipid Monolayers'rBiochemistry.  28:7. 1989
    1989 Infrared spectroscopic investigations of pulmonary surfactant. Surface film transitions at the air-water interface and bulk phase thermotropismBiophysical Journal.  56:1173-1181. 1989
    1989 Quantitative Determination of Conformational Disorder in the Acyl Chains of Phospholipid Bilayers by Infrared SpectroscopyBiochemistry.  28:8934-8939. 1989
    1988 Phospholipids chiral at phosphorus. Characterization of the sub-gel phase of thiophosphatidylcholines by use of X-ray diffraction, phosphorus-31 nuclear magnetic resonance, and Fourier transform infrared spectroscopy.Biochemistry.  27:4625-4631. 1988
    1988 Emerging techniques in biophysical FT-IR.Analytical Chemistry.  60:269A-278A. 1988
    1988 Design and interfacing of an automated Langmuir-type film balance to an FT-IR spectrometerApplied Spectroscopy.  42:1289-1293. 1988
    1988 In Situ FT-IR Investigation of Phospholipid Monolayer Phase Transitions at the Air-Water InterfaceJournal of the American Chemical Society.  110:712-718. 1988
    1988 In situ FT-IR studies of langmuir-blodgett monolayers 1988
    1987 BIOLOGICAL FTIR SPECTROSCOPY-AN OVERVIEWAmerican Biotechnology Laboratory.  5:10. 1987
    1986 Conformations of signal peptides induced by lipids suggest initial steps in protein exportScience.  233:206-208. 1986
    1986 Quantitative external reflection infrared spectroscopic analysis of insoluble monolayers spread at the air-water interfaceThe Journal of physical chemistry.  90:1373-1379. 1986
    1985 Infrared characterization of conformational differences in the lamellar phases of 1,3-dipalmitoyl-sn-glycero-2-phosphocholine 1985
    1985 Characterization of cooperative conformational transitions by Fourier transform infrared spectroscopy: application to phospholipid binary mixturesCanadian Journal of Chemistry.  63:1925-1932. 1985
    1985 In situ measurement of the infrared spectra of insoluble monolayers at the air-water interfaceThe Journal of physical chemistry.  89:3195-3197. 1985
    1984 Fourier transform infrared spectroscopic studies of lipid-protein interaction in native and reconstituted sarcoplasmic reticulum 1984
    1984 Calorimetric and fourier transform infrared spectroscopic studies on the interaction of glycophorin with phosphatidylserine/dipalmitoylphosphatidylcholine-d62 mixtures 1984
    1984 Infrared spectroscopic evidence for intermolecular hydrogen bonding in O-phospho-l-serine. A model for an anionic lipid headgroupBBA - Lipids and Lipid Metabolism.  792:182-185. 1984
    1984 Interaction of Glycophorin with Phosphatidylserine: A Fourier Transform Infrared InvestigationBiochemistry.  23:1498-1504. 1984
    1983 Fourier Transform Infrared Spectroscopic Studies of the Effect of Calcium Ions on PhosphatidylserineBiochemistry.  22:6318-6325. 1983
    1983 Interaction of Dipalmitoylphosphatidylcholine and Dimyristoylphosphatidylcholine-d54 Mixtures with Glycophorin. A Fourier Transform Infrared InvestigationBiochemistry.  22:1170-1177. 1983
    1982 DL Dorset and WA Pangborn, Ether linkages in glycerolipid~ Their effect on long chain packing 1 HL Casal, DG Cameron, HC Jarrell, ICP Smith and HH Mantseh, Lipid phase transitions in fatty acid-homogeneous membranes of Acholeplasma laidlawii B. 17 A. Liar and MB Goren, 3', 6'. Anhydro. 6-O-corynomycoloyl trehalose: synthesis andChemistry and Physics of Lipids.  30:403-404. 1982
    1982 Monomer-to-Micelle Transition of Dihexanoylphosphatidylcholine: 13C NMR and Raman StudiesJournal of the American Chemical Society.  104:430-438. 1982
    1982 Order and fluidity in the terminal methyl region of dipalmitoyl phosphatidylcholine multibilayers: A comparison of raman and 2H-NMR spectroscopyChemistry and Physics of Lipids.  30:287-295. 1982
    1982 Raman and Fourier Transform Infrared Spectroscopic Studies of the Interaction between Glycophorin and DimyristoylphosphatidylcholineBiophysical Journal.  37:83-84. 1982
    1981 Raman and Fourier Transform Infrared Spectroscopic Studies of the Interaction between Glycophorin and DimyristoylphosphatidylcholineBiochemistry.  20:6699-6706. 1981


    Year Title Altmetric
    2018 FT-IR studies of molecular conformation in biological membranes.  53-86. 2018
    1997 Determination of Microdomain Size in Langmuir-Blodgett Films of Binary Mixtures by IR Spectroscopy and Atomic Force Microscopy.  683-685. 1997
    1997 Internal Reflectance IR Spectroscopy of Langmuir Monolayer Films at the Air-Water Interface.  679-681. 1997
    1997 Structural Characterization of Supported Planar Bilayers by Using Infrared Spectroscopy and Atomic Force Microscopy.  455-457. 1997
    1997 The phase behavior of binary lipid monolayers as determined by surface chemistry and IR spectroscopy.  687-689. 1997
    1991 Monolayer structure at gas-liquid and gas solid interfaces-infrared reflectance spectroscopy as a probe.  192-207. 1991

    Research Overview

  • Our research interests are in the applications of nanoscience and nanotechnology to bioanalytical problems. We are currently interested in the development of new surface chemistry and surface spectroscopic methods for nano-optical detection in biomedical diagnostics. In particular, we are developing new nanofabrication and analysis methods that use surface-enhanced Raman spectroscopy (SERS) as the optical read-out technique to provide specific and quantitative information about biomedically important pathogens, including viruses and bacteria. We are developing new multivariate computational methods for the analysis of vibrational spectra. Our research interests also span a range of topics, especially the use of vibrational spectroscopy for the study of molecularly engineered surfaces for potential applications such as sensor or biomaterials, as well as the spectroscopic and microscopic imaging of surfaces and interfaces.
  • Teaching Overview

  • Quantitative analysis and analytical chemistry. Undergraduate and graduate-level instrumental analysis and spectroscopy. Development of computer applications for undergraduate learning in analytical chemistry. Development of integrated analytical and physical chemistry laboratory experiments. Biophysical chemistry. Nanoscience. Biochemical and biomedical applications of nanoscience
  • Education And Training

  • Doctor of Philosophy in Chemistry, Rutgers University : Newark 1983
  • National Research Council of Canada Division of Chemistry, Postdoctoral Fellowship 1984
  • Full Name

  • Richard Dluhy