Positions

Overview

  • Purnima Singh, MSc, PhD, MSPH joined UAB in 2015 as a member of The Institute for Cancer Outcomes and Survivorship. She is an Associate Professor in the Division of Pediatric - Hematology-Oncology. Following a PhD in Molecular Biology and Biotechnology from G. B. Pant University, India, Dr. Singh completed her postdoctoral training at City of Hope Comprehensive Cancer Center, CA in DNA Replication and Repair (Dr. Binghui Shen) and Developmental Epigenetics (Dr. Piroska Szabo). She was a Research faculty at the Dept. of Population Sciences at City of Hope. Her current goal is to understand the molecular and epigenetic basis of adverse outcomes in childhood cancer survivors by following an integrative genomics approach, to allow for targeted therapy, thus reducing morbidity in children with cancer.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2022 Genome-Wide Association Study Identifies ROBO2 as a Novel Susceptibility Gene for Anthracycline-Related Cardiomyopathy in Childhood Cancer Survivors.Journal of Clinical Oncology.  JCO2201527. 2022
    2022 Genome-wide variants and polygenic risk scores for cognitive impairment following blood or marrow transplantationBone Marrow Transplantation.  57:925-933. 2022
    2022 Immunogenicity and safety of the human papillomavirus vaccine in young survivors of cancer in the USA: a single-arm, open-label, phase 2, non-inferiority trialLancet Child and Adolescent Health.  6:38-48. 2022
    2021 EHMT2 suppresses the variation of transcriptional switches in the mouse embryoPLoS Genetics.  17. 2021
    2021 EHMT2 Controls Transcriptional Noise and the Developmental Switch after Gastrulation in the Mouse Embryo 2021
    2021 Prenatal correction of IGF2 to rescue the growth phenotypes in mouse models of Beckwith-Wiedemann and Silver-Russell syndromesCell Reports.  34. 2021
    2020 Association of GSTM1 null variant with anthracycline-related cardiomyopathy after childhood cancer—A Children's Oncology Group ALTE03N1 reportCancer.  126:4051-4058. 2020
    2020 GSTM1 null variant associated with anthracycline-related cancer in pediatric cancer.Cancer.  126:3926-3928. 2020
    2020 Genome Wide Association Study (GWAS) of cognitive impairment after blood or marrow transplantation (BMT) for hematologic malignancy.Journal of Clinical Oncology.  38:1536-1536. 2020
    2020 Clinical and genetic risk prediction of cognitive impairment after blood or marrow transplantation for hematologic malignancyJournal of Clinical Oncology.  38:1312-1321. 2020
    2020 Isolation of DNA-free RNA from human bone marrow mononuclear cells: Comparison of laboratory methods 2020
    2019 A novel association between GSTM1 null variant and anthracycline-induced cardiac dysfunction (ACD) in childhood cancer survivors (CCS): A COG ALTE03N1 report.Journal of Clinical Oncology.  37:10030-10030. 2019
    2019 Candidate SNPs enhance prediction of cognitive impairment after blood or marrow transplantation (BMT) for hematologic malignancy (HM).Journal of Clinical Oncology.  37:11520-11520. 2019
    2019 Risk prediction of anthracycline-related cardiomyopathy (AC) in childhood cancer survivors (CCS): A COG-ALTE03N1 and CCSS report.Journal of Clinical Oncology.  37:10015-10015. 2019
    2018 Factors associated with physical inactivity in adult breast cancer survivors—A population-based studyCancer Medicine.  7:6331-6339. 2018
    2017 Clinical and genetic risk prediction of subsequent CNS tumors in survivors of childhood cancer: A report fromthe COG ALTE03N1 studyJournal of Clinical Oncology.  35:3688-3696. 2017
    2016 Single Nucleotide Polymorphisms (SNPs) Associated with Cognitive Impairment in Patients Treated with Hematopoietic Cell Transplantation (HCT): A Longitudinal StudyBlood.  128:824-824. 2016
    2016 High type I error and misrepresentations in search for transgenerational epigenetic inheritance: Response to Guerrero-BosagnaGenome Biology.  17. 2016
    2016 CELF4 variant and anthracycline-related cardiomyopathy: A children's oncology group genome-wide association studyJournal of Clinical Oncology.  34:863-870. 2016
    2015 Draft genome of Cryobacterium sp. MLB-32, an obligate psychrophile from glacier cryoconite holes of high ArcticMarine Genomics.  21:25-26. 2015
    2015 CELF4 variant and Anthracycline-related Cardiomyopathy (anth-card) – A COG Study (ALTE03N1).Journal of Clinical Oncology.  33:10066-10066. 2015
    2015 Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogrammingGenome Biology.  16. 2015
    2014 Characterization of the imprinting signature of mouse embryo fibroblasts by RNA deep sequencingNucleic Acids Research.  42:1772-1783. 2014
    2013 De Novo DNA Methylation in the Male Germ Line Occurs by Default but Is Excluded at Sites of H3K4 MethylationCell Reports.  4:205-219. 2013
    2012 More than insulator: Multiple roles of CTCF at the H19-Igf2 imprinted domainFrontiers in Genetics.  3. 2012
    2012 Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domainsMethods in Molecular Biology.  925:159-172. 2012
    2012 Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.Methods in Molecular Biology.  925:159-172. 2012
    2011 Chromosome-wide analysis of parental allele-specific chromatin and DNA methylationMolecular and Cellular Biology.  31:1757-1770. 2011
    2011 Effects of endocrine disruptors on imprinted gene expression in the mouse embryoEpigenetics.  6:937-950. 2011
    2011 MIRA-SNuPE, a quantitative, multiplex method for measuring allele-specific DNA methylationEpigenetics.  6:212-223. 2011
    2010 Coordinated allele-specific histone acetylation at the differentially methylated regions of imprinted genesNucleic Acids Research.  38:7974-7990. 2010
    2010 CTCF-dependent chromatin bias constitutes transient epigenetic memory of the mother at the H19-igf2 imprinting control region in prospermatogoniaPLoS Genetics.  6. 2010
    2010 Complete biallelic insulation at the H19/Igf2 imprinting control region position results in fetal growth retardation and perinatal lethalityPLoS One.  5:1-11. 2010
    2010 Allele-specific H3K79 di- versus trimethylation distinguishes opposite parental alleles at imprinted regionsMolecular and Cellular Biology.  30:2693-2707. 2010
    2009 The 3′-flap pocket of human flap endonuclease 1 is critical for substrate binding and catalysisJournal of Biological Chemistry.  284:22184-22194. 2009
    2008 Overexpression and hypomethylation of Flap endonuclease 1 gene in breast and other cancersMolecular Cancer Research.  6:1710-1717. 2008
    2008 Comprehensive Mapping of the C-Terminus of Flap Endonuclease-1 Reveals Distinct Interaction Sites for Five Proteins That Represent Different DNA Replication and Repair PathwaysJournal of Molecular Biology.  377:679-690. 2008
    2007 Fen1 mutations result in autoimmunity, chronic inflammation and cancersNature Medicine.  13:812-819. 2007
    2007 Concerted action of exonuclease and gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG) n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragmentsJournal of Biological Chemistry.  282:3465-3477. 2007
    2005 Characterization of the 3′ → 5′ exonuclease activity found in human nucleoside diphosphate kinase 1 (NDK1) and several of its homologuesBiochemistry.  44:15774-15786. 2005
    2005 Multiple but dissectible functions of FEN-1 nucleases in nucleic acid processing, genome stability and diseasesBioEssays.  27:717-729. 2005
    2005 Dual negative regulatory mechanisms of RecX on RecA functions in radiation resistance, DNA recombination and consequent genome instability in Deinococcus radioduransDNA Repair.  4:671-678. 2005
    2003 Enhanced transformation of plant cells following co-bombardment of VirE2 protein of Agrobacterium tumefaciens with DNA substrateCurrent Science.  85:1343-1347. 2003
    2003 Transient expression of foreign genes in mature wheat embryo explants following particle bombardmentPhysiology and Molecular Biology of Plants.  9:217-223. 2003

    Chapter

    Year Title Altmetric
    2007 Nucleases in DNA repair, replication and recombination: Flap endonuclease-1 as a paradigm.  267-300. 2007

    Research Overview

  • As an integral member of the Institute for Cancer Outcomes and Survivorship at UAB, my goal is to understand the molecular and epigenetic basis of adverse outcomes in childhood cancer survivors. Anthracycline-related cardiomyopathy and congestive heart failure is the leading cause of late morbidity in survivors of childhood cancers. There is a dose-dependent association between anthracycline exposure and cardiomyopathy risk, suggesting that genetic predisposition possibly modifies this association. I am following an integrative genomics approach to understand whether genetic and epigenetic predisposition can place childhood cancer survivors at an increased risk for developing treatment-related complications and therefore allow for targeted therapy, thus reducing morbidity in children with cancer.
  • Investigator On

  • (PQB-1) Telomere Maintenance Defects and Thyroid Second Cancer in Childhood Cancer Survivors  awarded by Baylor College of Medicine
  • A Comprehensive Investigation of Outcome Disparities in Children, Adolescents and Young Adults with Cancer  awarded by KAUL PEDIATRIC RESEARCH INSTITUTE
  • BMT Survivor Study-2 (BMTSS-2)  awarded by National Cancer Institute/NIH/DHHS
  • COG PCR - Per Case Reimbursement NIH National Clinical Trials Network (NCTN) Grant (U10CA180886) Successor to NIH COG Chair Grant (U10CA098543)  awarded by CHILDREN'S HOSPITAL (PHILADELPHIA)
  • COG PCR - Per Case Reimbursement NIH National Clinical Trials Network (NCTN) Grant (U10CA180886) Successor to NIH COG Chair Grant (U10CA098543)  awarded by CHILDREN'S HOSPITAL (PHILADELPHIA)
  • COG PCR - Per Case Reimbursement NIH National Clinical Trials Network (NCTN) Grant (U10CA180886) Successor to NIH COG Chair Grant (U10CA098543)  awarded by CHILDREN'S HOSPITAL (PHILADELPHIA)
  • City of Hope Lymphoma SPORE  awarded by BECKMAN RESEARCH INSTITUTE (CITY OF HOPE)
  • Enhancing 6MP Adherence in Adolescents with ALL: Strategy for Dissemination  awarded by RALLY FOUNDATION
  • Impact of Cognitive Training on Cognition after Allogeneic Hematopoietic Cell Transplantation - A Randomized Wait-listed Clinical Trial  awarded by National Marrow Donor Program
  • Mitigating Long-term Treatment-related Morbidity in Childhood Cancer Survivors  awarded by National Cancer Institute/NIH/DHHS
  • Novel Methods to Detect Gene-Environment Interactions in Treatment-Related Brain Tumors Among Childhood Cancer Survivors  awarded by University of Wisconsin-Milwaukee
  • Per Case Reimbursement (PCR)- NIH National Clinical Trails Network (NCTN) Grant (U10CA180886) -PHI/COG  awarded by PUBLIC HEALTH INSTITUTE (PHI)
  • Per Case Reimbursement COG NCORP Research Base  awarded by CHILDREN'S HOSPITAL (PHILADELPHIA)
  • Per Case Reimbursement: COG NCORP Research Base  awarded by CHILDREN'S HOSPITAL (PHILADELPHIA)
  • Predicting the Risk of Heart Failure in Breast Cancer Survivors  awarded by BREAST CANCER RESEARCH FOUNDATION OF ALABAMA
  • St. Baldrick's Foundation Supplemental Reimbursement  awarded by CHILDREN'S HOSPITAL (PHILADELPHIA)
  • The Who and Why of Anthracycline-related Cardiotoxicity in Childhood Cancer Survivors  awarded by Leukemia and Lymphoma Society
  • Understanding Outcome Disparities in AYA with ALL  awarded by RALLY FOUNDATION
  • Understanding the Molecular Underpinnings of Anthracycline-related Cardiomyopathy in Childhood Cancer Survivors  awarded by V FOUNDATION FOR CANCER RESEARCH
  • Education And Training

  • Beckman Research Institute, City of Hope Molecular and Cellular Biology, Postdoctoral Research
  • Beckman Research Institute, City of Hope Radiation Biology, Postdoctoral Research
  • Doctor of Philosophy in Molecular Biology, 2001
  • Full Name

  • Purnima Singh
  • Fax

  • 205-638-2121