Because management of the fisheries worldwide has not been successful, intensive efforts are being developed for aquaculture to meet the demand for sea urchins. The Gulf of Mexico does not have an established fishery for sea urchins, but one species shows great potential for a fishery and for aquaculture. Lytechinus variegatus exhibits rapid growth, and individuals reach gonadal maturity in less than 1 year. We measured roe production in adults fed two formulated diets for a period of 10 weeks in the laboratory. Gonads increased in size in individuals fed either diet, although roe production was less than the maximal roe production observed in natural populations. Gonad color varied with diet, which was attributed to differences in the primary ingredients of each feed. Proximate composition of the gonads indicated that diet had no effect on carbohydrate and lipid levels, however, protein levels increased with both diets.