Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase α- and β-subunits, and identification of β′-, a putative β-subunit isozyme produced by alternative splicing of the β-subunit gene

Academic Article

Abstract

  • We have characterized overlapping cDNA clones encoding cGMP phosphodiesterase (PDE) α- and β-subunits of mouse retinal rod photoreceptors. The open reading frames predict an α-subunit of 100 kDa (856 residues), and a β-subunit of 99 kDa (853 residues). Sequence analysis of two of twelve β-subunit clones predicts the presence in the retina of an additional PDE, termed β, which is generated by alternative splicing of the β-subunit gene, β differs from β only at the C-terminus being 55 residues shorter and lacking the Caax motif found at the C-termini of both the α- and β-subunits. A 300 residue segment thought to contain the active site is present in the C-terminal half of α, β and β. © 1991.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Baehr W; Champagne MS; Lee AK; Pittler SJ
  • Start Page

  • 107
  • End Page

  • 114
  • Volume

  • 278
  • Issue

  • 1