Potassium channels in colonic crypts

Academic Article

Abstract

  • A characteristic feature of intestinal epithelia is their ability to secrete chloride (Cl ) a process that occurs mainly in intestinal crypts and is the critical transport event in secretory diarrhoea. Increased potassium (K ) channel activity in the basolateral membrane has an important role in the Cl-secretory process by hyperpolarising the cell and maintaining a favourable electrochemical driving force for Cl exit at the apical membrane. We have shown, using patch-clamp techniques, that the basolateral membrane of human colonic crypt cells contains low conductance K channels that are voltage and calcium (Ca ) sensitive and blocked by barium (Ba ). These K channels are regulated by cytosolic cyclic adenosine monophosphate (cAMP) and Ca , intracellular second messengers that also stimulate Cl secretion. This population of human intestinal K channels may be a target for the pharmacological control of Cl secretory diarrhoea. © 1994. - + - + 2+ 2+ + 2+ - + -
  • Digital Object Identifier (doi)

    Author List

  • Sandle GI; McNicholas CM; Lomax RB
  • Start Page

  • 23
  • End Page

  • 25
  • Volume

  • 343
  • Issue

  • 8888