Molecular analysis of t(11;19) breakpoints in childhood acute leukemias

Academic Article

Abstract

  • MLL is fused to ENL or ELL in acute leukemias that contain t(11;19)(q23;p13). Although ENL and ELL localize to chromosome 19, bands p13.3 and p13.1, respectively, these breakpoints are not always readily distinguished by standard cytogenetics. We therefore used reverse transcriptase-polymerase chain reaction (RT-PCR) assays to analyze 26 cases of childhood acute leukemia containing t(11;19) to determine the frequencies of ENL and ELL involvement. All 17 cases of acute lymphoblastic leukemia (ALL) had MLL/ENL fusion transcripts. By contrast, of the 9 cases of acute myeloid leukemia (AML) analyzed, 6 had MLL/ENL fusions, 2 had MLL/ELL fusions, and 1 case had no RT-PCR-detectable MLL fusion mRNA. These data suggest that the majority of 11;19 translocations involve ENL, whereas involvement of ELL is relatively uncommon in childhood acute leukemia and may be restricted to AML.
  • Published In

  • Blood  Journal
  • Digital Object Identifier (doi)

    Author List

  • Rubnitz JE; Behm FG; Curcio-Brint AM; Pinheiro VRP; Carroll AJ; Raimondi SC; Shurtleff SA; Downing JR
  • Start Page

  • 4804
  • End Page

  • 4808
  • Volume

  • 87
  • Issue

  • 11