Direct characterization of transcription elongation by RNA polymerase I

Academic Article


  • RNA polymerase I (Pol I) transcribes ribosomal DNA and is responsible for more than 60% of transcription in a growing cell. Despite this fundamental role that directly impacts cell growth and proliferation, the kinetics of transcription by Pol I are poorly understood. This study provides direct characterization of S. Cerevisiae Pol I transcription elongation using tethered particle microscopy (TPM). Pol I was shown to elongate at an average rate of approximately 20 nt/s. However, the maximum speed observed was, in average, about 60 nt/s, comparable to the rate calculated based on the in vivo number of active genes, the cell division rate and the number of engaged polymerases observed in EM images. Addition of RNA endonucleases to the TPM elongation assays enhanced processivity. Together, these data suggest that additional transcription factors contribute to efficient and processive transcription elongation by RNA polymerase I in vivo.
  • Published In

  • PLoS One  Journal
  • Digital Object Identifier (doi)

    Author List

  • Ucuncuoglu S; Engel KL; Purohit PK; Dunlap DD; Schneider DA; Finzi L
  • Volume

  • 11
  • Issue

  • 7