A novel active DNA topoisomerase I in Leishmania donovani

Academic Article


  • A common feature shared by type I DNA topoisomerases is the presence of a "serine, lysine, X, X, tyrosine" motif as conventional enzyme active site. Preliminary data have shown that Leishmania donovani DNA topoisomerase I gene (LdTOP1A) lacked this conserved motif, giving rise to different theories about the reconstitution of an active DNA topoisomerase I in this parasite. We, herein, describe the molecular cloning of a new DNA topoisomerase I gene from L. donovani (LdTOP1B) containing the highly conserved serine, lysine, X, X, tyrosine motif. DNA topoisomerase I activity was detected only when both genes (LdTOP1A and LdTOP1B) were co-expressed in a yeast expression system, suggesting the existence of a dimeric DNA topoisomerase I in Leishmania parasites.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Villa H; Marcos ARO; Reguera RM; Balaña-Fouce R; García-Estrada C; Pérez-Pertejo Y; Tekwani BL; Myler PJ; Stuart KD; Bjornsti MA
  • Start Page

  • 3521
  • End Page

  • 3526
  • Volume

  • 278
  • Issue

  • 6