Rapid and selective remodeling of a positioned nucleosome during the induction of IL-12 p40 transcription

Academic Article

Abstract

  • Nucleosomes are important for gene regulation, but comprehensive studies of nucleosome positioning, remodeling, and transcription factor binding at inducible mammalian promoters have not been reported. We have analyzed the IL-12 p40 promoter, which is induced in macrophages by bacterial products. High-resolution micrococcal nuclease analyses revealed that a positioned nucleosome, nucleosome 1, spans the promoter, with three positioned nucleosomes further upstream. Upon activation, nucleosome 1 was rapidly and selectively remodeled in a protein synthesis-dependent manner. In primary macrophages, IFNγ synergistically enhanced p40 expression, but little effect on remodeling or promoter occupancy was observed. These results suggest that remodeling complexes are selectively targeted to a single, promoter- encompassing nucleosome and that IFNγ influences an event that is independent or downstream of remodeling.
  • Published In

  • Immunity  Journal
  • Digital Object Identifier (doi)

    Author List

  • Weinmann AS; Plevy SE; Smale ST
  • Start Page

  • 665
  • End Page

  • 675
  • Volume

  • 11
  • Issue

  • 6