Identification and characterization of an apparent germline set of auto-anti-idiotypic regulatory B lymphocytes

Academic Article

Abstract

  • By fusing BALB/c splenic lymphocytes from mice immunized with phosphorylcholine (PC) to an immunoglobulin nonproducing plasmacytoma cell line, a B cell hybridoma was isolated (MM-60) that has been shown by multiple criteria to produce a bona fide auto-anti-(anti-T15 idiotype) antibody. In vivo administration of MM-60 antibody suppressed T15+anti-PC antibody production in an idiotope-specific manner by activation of an intervening set of anti-T15 B cells. These T15-specific B cells i) appeared to express germline-encoded variable region gene products, ii) developed in parallel to, but independent of, T15+B cells, and iii) suppressed the anti-PC response in a T cell-independent fashion. Variants of T15+anti-PC B cells possessing aberrant immunoglobulin heavy chain GD region structure escaped from the suppression by this anti-T15 B cell set, suggesting that a function of the heavy chain D region may be to contribute to the formation of molecular target sites for idiotype-directed regulatory cells and/or antibodies. The indigenous nature of these particular populations of antiidiotypic and anti-(anti-idiotypic) B cells and the ability of their immunoglobulin products to regulate antigen-specific B cells in vivo provides strong supportive evidence for the significant role idioptype-directed network interactions play in regulating specific antibody production during a normal immune response.
  • Published In

    Author List

  • Pollok BA; Kearney JF
  • Start Page

  • 44
  • End Page

  • 121
  • Volume

  • 132
  • Issue

  • 1