Complement in BuB/BnJ mice revisited: Serum C3 levels and complement opsonic activity are not elevated

Academic Article


  • With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels comparable to those seen in C57BL/6, Balb/c and several other strains of inbred mice. More importantly, using bacteria as a substrate for activation and deposition of complement as a direct biological assay to assess serum complement opsonic activity, we found that BuB/BnJ mice do not have elevated complement activity compared to other inbred mouse strains. In contrast hemolytic assays indicate BuB/BnJ sera to be most active. These results indicate that accepted views regarding mouse serum complement activity need to be re-examined. © 2005 Elsevier Ltd. All rights reserved.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Osmers I; Szalai AJ; Tenner AJ; Barnum SR
  • Start Page

  • 1722
  • End Page

  • 1725
  • Volume

  • 43
  • Issue

  • 10