Muscarinic receptor stimulation modulates the effect of halothane on Mn2+ influx in airway smooth muscle.

Academic Article

Abstract

  • Prior studies suggest that the mechanism of action by which halothane relaxes airway smooth muscle depends on the contractile state of the cell. We hypothesized that halothane would inhibit the influx of Ca2+ into canine airway smooth muscle cells during submaximal, but not maximal, muscarinic stimulation. This hypothesis was tested by using the rate of quenching of fura 2 fluorescence by Mn2+ in strips of canine tracheal smooth muscle as an index of Ca2+ influx. Acetylcholine (ACh) produced a dose-dependent increase in Mn2+ influx. Halothane (0.64 +/- 0.05 microM) significantly decreased Mn2+ influx and intracellular Ca2+ concentration when added to strips stimulated with a submaximal concentration of ACh (0.3 microM) but had no effect on Mn2+ influx or intracellular Ca2+ concentration during maximal stimulation with ACh (100 microM). Similar results were observed when the strips were treated with verapamil. These results demonstrate that anesthetic effects on Ca2+ homeostasis in intact canine tracheal smooth muscle cells may be critically modulated by receptor-linked mechanisms.
  • Authors

    Published In

    Keywords

  • Acetylcholine, Analysis of Variance, Animals, Calcium, Calcium Channels, Dogs, Female, Halothane, Isometric Contraction, Kinetics, Male, Manganese, Muscle, Smooth, Nifedipine, Potassium, Receptors, Muscarinic, Trachea, Verapamil
  • Digital Object Identifier (doi)

    Author List

  • Warner DO; Jones KA; Lorenz RR; Pabelick CM
  • Start Page

  • C868
  • End Page

  • C873
  • Volume

  • 273
  • Issue

  • 3 Pt 1