cGMP modulation of Ca2+ sensitivity in airway smooth muscle.

Academic Article

Abstract

  • A beta-escin-permeabilized canine tracheal smooth muscle preparation was used to test the hypothesis that cGMP decreases Ca2+ sensitivity in airway smooth muscle primarily by inhibiting the membrane receptor-coupled mechanisms that regulate Ca2+ sensitivity and not by inhibiting Ca2+/calmodulin activation of the contractile proteins. 8-Bromo-cGMP (100 microM) had no effect on the free Ca2+ concentration-response curves generated in the absence of muscarinic receptor stimulation. In the presence of 100 microM ACh plus 10 microM GTP, 8-bromo-cGMP (100 microM) caused a rightward shift of the free Ca2+ concentration-response curve, significantly increasing the EC50 for free Ca2+ from 0.35 +/- 0.03 to 0.75 +/- 0.06 microM; this effect of 8-bromo-cGMP was concentration dependent from 1 to 100 microM. 8-Bromo-cGMP (100 microM) decreased the level of regulatory myosin light chain (rMLC) phosphorylation for a given cytosolic Ca2+ concentration but had no effect on the amount of isometric force produced for a given level of rMLC phosphorylation. These findings suggest that cGMP decreases Ca2+ sensitivity in canine tracheal smooth muscle primarily by inhibiting the membrane receptor-coupled mechanisms that modulate the relationship between cytosolic Ca2+ concentration and rMLC phosphorylation.
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    Published In

    Keywords

  • Animals, Calcium, Cyclic GMP, Dogs, Female, In Vitro Techniques, Isometric Contraction, Male, Muscle, Smooth, Myosin Light Chains, Osmolar Concentration, Phosphorylation, Receptors, Muscarinic, Stimulation, Chemical, Trachea
  • Digital Object Identifier (doi)

    Author List

  • Jones KA; Wong GY; Jankowski CJ; Akao M; Warner DO
  • Start Page

  • L35
  • End Page

  • L40
  • Volume

  • 276
  • Issue

  • 1