Origin of CD4-CD8-B220+ T cells in MRL-lpr/lpr mice: Clues from a T cell receptor β transgenic mouse

Academic Article

Abstract

  • Abnormal development of T cells in the thymus is thought to be related to autoimmune disease and the expansion of the unusual CD4-CD8-B220+ peripheral T cell subset that results in lymphadenopathy in MRL-lpr/lpr mice. Although we and others have previously shown that rearranged TCR- transgenes alter T cell development in the thymus and abrogate lymphoproliferative disease in lpr mice, the origin and developmental pathway of the LN CD4- CD8-B220+ T cells has not been fully elucidated. We therefore undertook the systematic analysis of the effect of a TCR-β transgene on the production and differentiation of (lymph node) LN T cells and the production, differentiation, and release of thymocyte T cell populations. In nontransgenic mice, there was increased proliferation of CD4-CD8-B220+ T cells in the LN of adult MRL-lpr/lpr mice compared to MRL-+/+ mice, as measured by in vivo BrdU labeling. These proliferating LN T cells were greatly reduced by thymectomy of adult MRL-lpr/lpr mice 1 wk before bromodeoxyurindine labeling, indicating that recent thymic emigrants or factors were required to sustain proliferation. In the thymus, there was increased production and accumulation of CD4+CD8+TCR(dull) thymocytes in nontransgenic MRL-lpr/lpr compared to MRL-+/+ mice. As the rate of maturation from CD4+CD8+TCR(dull) to CD4+CD8+TCR(bright) was the same (6%) in both MRL-Ipr/Ipr MRL-lpr/lpr and MRL-+/+ mice, the accumulation of the immature population in the MRL-lpr/lpr mice could not be due to a maturation defect. However, there was a decrease in apoptosis and intrathymic death of CD4+CD8+TCR(dull) thymocytes in MRL-lpr/lpr compared to MRL-+/+ mice. Introduction of the TCR-β transgene into lpr/lpr mice normalized the proliferation of T cells in the LN. In the thymus, the TCR-β transgene resulted in a dramatic increase in maturation efficiency and a reduction in apoptosis in MRL-+/+ mice. These data suggest that the TCR transgene inhibits lymphoproliferation by reducing the production of 'neglected' CD4+CD8+TCR(dull) thymocytes that will undergo Fas Ag-mediated apoptosis. They further suggest that in lpr mice, which express a mutated Fas Ag, the 'neglected' thymocytes are able to continually escape to the periphery, where they proliferate.
  • Published In

    Author List

  • Zhou T; Bluethmann H; Eldridge J; Berry K; Mountz JD
  • Start Page

  • 3651
  • End Page

  • 3667
  • Volume

  • 150
  • Issue

  • 8 I