Phosphatidylinositol 4,5-bisphosphate (PIP2) stimulates the electrogenic Na/HCO3 cotransporter NBCe1-A expressed in Xenopusoocytes

Academic Article

Abstract

  • Bicarbonate transporters are regulated by signaling molecules/ ions such as protein kinases, ATP, and Ca . While phospholipids such as PIP can stimulate Na-H exchanger activity, little is known about phospholipid regulation of bicarbonate transporters. We used the patch-clamp technique to study the function and regulation of heterologously expressed rat NBCe1-A in excised macropatches from Xenopus laevis oocytes. Exposing the cytosolic side of inside-out macropatches to a 5% CO /33 mM HCO - solution elicited a mean inward current of 14 pA in 74% of macropatches attached to pipettes (-Vp = -60 mV) containing a low-Na+, nominally HCO -free solution. The current was 80-90% smaller in the absence of Na , approximately 75% smaller in the presence of 200 μM DIDS, and absent in macropatches from H O-injected oocytes. NBCe1-A currents exhibited time-dependent rundown that was inhibited by removing Mg in the presence or absence of vanadate and F- to reduce general phosphatase activity. Applying 5 or 10 μM PIP (diC8) in the presence of HCO - induced an inward current in 54% of macropatches from NBC-expressing, but not H2O-injected oocytes. PIP -induced currents were HCO -dependent and somewhat larger following more NBCe1-A rundown, 62% smaller in the absence of Na , and 90% smaller in the presence of 200 μM DIDS. The polycation neomycin (250-500 μM) reduced the PIP -induced inward current by 69%; spermine (100 μM) reduced the current by 97%. Spermine, poly-D-lysine, and neomycin all reduced the baseline HCO -induced inward currents by as much as 85%. In summary, PIP stimulates NBCe1-A activity, and phosphoinositides are regulators of bicarbonate transporters. 2+ - + 2+ - + - 2 2 3 3 2 2 3 2 3 - 2 3 2
  • Digital Object Identifier (doi)

    Author List

  • Wu J; McNicholas CM; Bevensee MO
  • Start Page

  • 14150
  • End Page

  • 14155
  • Volume

  • 106
  • Issue

  • 33