CD8+ T-cell clones deficient in the expression of the CD45 protein tyrosine phosphatase have impaired responses to T-cell receptor stimuli.

Academic Article

Abstract

  • CD45 is a high-molecular-weight transmembrane protein tyrosine phosphatase expressed only by nucleated cells of hematopoietic origin. To examine function, mouse CD8+ cytolytic T-cell clones were derived that had a specific defect in the expression of CD45. Northern (RNA) blot analysis indicates that the CD45 deficiency is due to either a transcriptional defect or mRNA instability. The CD45-deficient cells were greatly diminished in their ability to respond to antigen. All functional parameters of T-cell receptor signalling analyzed (cytolysis of targets, proliferation, and cytokine production) were markedly diminished. A CD45+ revertant was isolated, and the ability to respond to antigen was restored. These results support a central and immediate role for this transmembrane protein tyrosine phosphatase in T-cell receptor signalling.
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    Keywords

  • Animals, Antigens, CD, Antigens, Differentiation, T-Lymphocyte, Blotting, Northern, CD8 Antigens, Cell Division, Cell Line, Clone Cells, Female, Flow Cytometry, Histocompatibility Antigens, Leukocyte Common Antigens, Lymphokines, Mice, Mice, Inbred CBA, Mice, Inbred DBA, Phosphoprotein Phosphatases, Protein Tyrosine Phosphatases, Receptors, Antigen, T-Cell, Signal Transduction, T-Lymphocytes, Cytotoxic
  • Digital Object Identifier (doi)

    Author List

  • Weaver CT; Pingel JT; Nelson JO; Thomas ML
  • Start Page

  • 4415
  • End Page

  • 4422
  • Volume

  • 11
  • Issue

  • 9