Regulation of β-actin gene transcription by insulin and phorbol esters

Academic Article


  • Insulin and the phorbol ester, phorbol 12-myristate 13-acetate, induce β-actin gene transcription in H4 cells. This occurred rapidly, with a maximum 15-fold stimulation following 15 min of insulin (5 × 10-9 M) or phorbol ester (1 μg/ml) exposure. The increase in β-actin transcription was transitory, returning to baseline within 120 min. Pretreatment of cells with phorbol ester for 24 h, reducing functional protein kinase C activity, abolished the ability of phorbol esters to increase β-actin transcription. When insulin was added to phorbol ester-pretreated cells the insulin-induced increase in β-actin transcription was reduced by 40-60%. These findings support our hypothesis that a common regulatory signal is utilized by both insulin and phorbol esters for the complete induction of specific genes. © 1992.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Messina JL; Weinstock RS
  • Start Page

  • 532
  • End Page

  • 535
  • Volume

  • 200
  • Issue

  • 2