Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

Academic Article

Abstract

  • Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.

    • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Southgate VJ; Steyn AJC; Pretorius IS; Van Vuuren HJJ

    • Start Page

  • 1253

    • End Page

  • 1258

    • Volume

  • 59

    • Issue

  • 4