Characterization of a mumps virus nucleocapsidlike particle

Academic Article


  • The nucleocapsid protein (NP) of mumps virus (MuV), a paramyxovirus, was coexpressed with the phosphoprotein (P) in Escherichia coli. The NP and P proteins form a soluble complex containing RNA. Under a transmission electron microscope, the NP-RNA complex appears as a nucleocapsidlike ring that has a diameter of approximately 20 nm with 13 subunits. There is a piece of single-stranded RNA with a length of 78 nucleotides in the NP-RNA ring. Shorter RNA pieces are also visible. The MuV NP protein may provide weaker protection of the RNA than the NP protein of some other negative-strand RNA viruses, reflecting the degree of NP protein association. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 19798416
  • Author List

  • Cox R; Green TJ; Qiu S; Kang J; Tsao J; Prevelige PE; He B; Luo M
  • Start Page

  • 11402
  • End Page

  • 11406
  • Volume

  • 83
  • Issue

  • 21