Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22

Academic Article


  • Packed and ready to go: A scaffold protein (SP) aids the assembly of Salmonella typhimuriam bacteriophage P22 into a capsid, with encapsulation of the SP. This natural process was exploited by using an engineered molecular system to fuse a fluorescent protein cargo (green in the picture) to a portion of the SP (yellow), which templated accurate spontaneous assembly. Heating of the capsids and treatment with thrombin released the SP but not the cargo. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 21407173
  • Author List

  • O'Neil A; Reichhardt C; Johnson B; Prevelige PE; Douglas T
  • Start Page

  • 7425
  • End Page

  • 7428
  • Volume

  • 50
  • Issue

  • 32