A potential estrogen mimetic effect of a bis(ethyl)polyamine analogue on estrogen receptor positive MCF-7 breast cancer cells

Academic Article

Abstract

  • BE-3-3-3-3 (1,15-(ethylamino)4,8,12-triazapentadecane) is a bis(ethyl)polyamine analogue under investigation as a therapeutic agent for breast cancer. Since estradiol (E 2) is a critical regulatory molecule in the growth of breast cancer, we examined the effect of BE-3-3-3-3 on estrogen receptor α (ERα) positive MCF-7 cells in the presence and absence of E 2. In the presence of E 2, a concentrationdependent decrease in DNA synthesis was observed using [ 3H]-thymidine incorporation assay. In the absence of E 2, low concentrations (2.5-10 μM) of BE-3-3-3-3 increased [ 3H]- thymidine incorporation at 24 and 48 h. BE-3-3-3-3 induced the expression of early response genes, c-myc and cfos, in the absence of E 2, but not in its presence, as determined by real-time quantitative polymerase chain reaction (qPCR). BE-3-3-3-3 had no significant effect on these genes in an ERα-negative cell line, MDA-MB-231. Chromatin immunoprecipitation assay demonstrated enhanced promoter occupation by either E 2 or BE-3-3-3-3 of an estrogenresponsive gene pS2/Tff1 by ERα and its co-activator, steroid receptor co-activator 3 (SRC-3). Confocal microscopy of BE-3-3-3-3-treated cells revealed membrane localization of ERα, similar to that induced by E 2. The failure of BE-3-3-3-3 to inhibit cell proliferation was associated with autophagic vacuole formation, and the induction of Beclin 1 and MAP LC3 II. These results indicate a differential effect of BE-3-3- 3-3 on MCF-7 cells in the absence and presence of E 2, and suggest that pre-clinical and clinical development of polyamine analogues might require special precautions and selection of sensitive subpopulation of patients. © Springer-Verlag 2011.
  • Authors

    Published In

  • Amino Acids  Journal
  • Digital Object Identifier (doi)

    Author List

  • Nayvelt I; John S; Hsu HC; Yang P; Liu W; Das G; Hyvönen MT; Alhonen L; Keinänen TA; Shirahata A
  • Start Page

  • 899
  • End Page

  • 911
  • Volume

  • 42
  • Issue

  • 2-3