In vitro-generated immune complexes containing galactose-deficient IgA1 stimulate proliferation of mesangial cells

Academic Article

Abstract

  • IgA nephropathy (IgAN) patients have elevated serum levels of immune complexes consisting of IgA1 with galactose-deficient hinge-region O-glycans (Gd-IgA1) and anti-glycan IgG. These immune complexes deposit in the kidney and activate mesangial cells. To confirm that the activity of these immune complexes depends on the interaction of Gd-IgA1 with anti-glycan IgG, we generated in vitro analogous immune complexes using Gd-IgA1 myeloma protein and anti-glycan IgG from cord blood of healthy women. The Gd-IgA1 and anti-glycan IgG from cord-blood serum formed IgA1-IgG immune complexes that resembled those in sera of patients with IgAN. Furthermore, the ability to activate cellular proliferation was dependent on a heat-sensitive serum factor. In summary, we developed a new protocol for in-vitro formation of IgA1-IgG immune complexes, thus providing a new tool for studies of the pathogenesis of IgAN. © 2012 Elsevier B.V.
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    Digital Object Identifier (doi)

    Author List

  • Yanagihara T; Brown R; Hall S; Moldoveanu Z; Goepfert A; Tomana M; Julian BA; Mestecky J; Novaka J
  • Start Page

  • 166
  • End Page

  • 172
  • Volume

  • 2