The Type 2 dUTPase of Bacteriophage PdblNM1 Initiates Mobilization of Staphylococcus aureus Bovine Pathogenicity Island 1

Academic Article

Abstract

  • Staphylococcus aureus pathogenicity islands (SaPIs) are genetic elements that are mobilized by specific helper phages. The initial step in mobilization is the derepression of the SaPI by the interaction of a phage protein with the SaPI master repressor Stl. Stl proteins are highly divergent between different SaPIs and respond to different phage-encoded derepressors. One such SaPI, SaPIbov1, is derepressed by the dUTPase (Dut) of bacteriophage 80α (Dut ) and its phage Pdbl11 homolog, Dut . We previously showed that SaPIbov1 could also be mobilized by phage PdblNM1, even though its dut gene is not homologous with that of 80α. Here, we show that PdblNM1 dut encodes a type 2 dUTPase (Dut ), which has an α-helical structure that is distinct from the type 1 trimeric, β-sheet structure of Dut . Deletion of dut abolishes the ability of PdblNM1 to mobilize SaPIbov1. Like Dut , Dut forms a direct interaction with SaPIbov1 Stl both in vivo and in vitro, leading to inhibition of the dUTPase activity and Stl release from its target DNA. This work provides novel insights into the diverse mechanisms of genetic mobilization in S. aureus. 80α 11 NM1 80α NM1 80α NM1
  • Digital Object Identifier (doi)

    Author List

  • Hill RLL; Dokland T
  • Start Page

  • 142
  • End Page

  • 152
  • Volume

  • 428