Pre-B cells: Bone marrow persistence in anti-μ-suppressed mice, conversion to B lymphocytes, and recovery after destruction by cyclophosphamide

Academic Article

Abstract

  • Chronic treatment of mice from birth with anti-μ antibodies aborts development of B lymphocytes and plasma cells. In these studies we show that bone marrow from anti-μ-treated mice contains a population of cells with cytoplasmic IgM, but which lack detectable cell-surface IgM. These cells are analogous to pre-B cells, defined in ontogenetic studies as the immediate precursors of B lymphocytes. Pre-B cells from bone marrow of anti-μ treated mice retain their functional integrity, as evidenced by their ability to give rise to sIgM+, LPS-responsive lymphocytes in culture. We also show that cyclophosphamide treatment destroys pre-B cells and that recovery of pre-B cells in bone marrow precedes the regeneration of sIgM+ B lymphocytes. Generation of B lymphocytes in adult mice apparently occurs exclusively in the bone marrow because induction of extramedullary hemopoiesis in spleen was not accompanied by the appearance of pre-B cells in the organ.
  • Author List

  • Burrows PD; Kearney JF; Lawton AR; Cooper MD
  • Start Page

  • 1526
  • End Page

  • 1531
  • Volume

  • 120
  • Issue

  • 5