Ten percent of normal B cells and plasma cells share A V(H) determinants) (J606-GAC) with a distinct subset of murine V(H)III plasmacytomas

Academic Article

Abstract

  • Our findings indicate that a subset of V(H)III antibodies, which we refer to as J606-GAC, contains a determinant(s) that is present on 5 to 15% of normal splenic B cells and plasma cells as detected by immunofluorescence. This subpopulation is detected by purified antibody, 0-1, which was prepared against a murine anti-group A carbohydrate (anti-GAC) hybridoma antibody. The J606-GAC subset includes the β2,1 fructosan myelomas J606, EPC109, W3082, ABPC4, and UPC61, as well as 13 anti-GAC hybridomas. The 0-1 antiserum failed to react with hybridoma and myeloma immunoglobulins from murine V(H) groups I and II or other V(H)III antibodies. By Western blot analysis, it was observed to react with isolated heavy, but not light, chains of J606-GAC-bearing antibodies. 0-1 failed to react with myelomas XRPC44 and J539, which have the same J region as J606 but a very different V(H) region. These observations indicate that 0-1 is detecting a V(H) region determinant. The J606-GAC marker recognized by 0-1 was expressed as early as 4 days after birth and was expressed at similar frequencies in germfree and conventional mice. Immunoprecipitation of both surface and biosynthetically labeled proteins from spleen cells or J606-GAC-positive hybridoma cell lines, respectively, confirmed that 0-1 was recognizing an immunoglobulin determinant.
  • Published In

    Author List

  • Basta P; Kubagawa H; Kearney JF; Briles DE
  • Start Page

  • 2423
  • End Page

  • 2428
  • Volume

  • 130
  • Issue

  • 5