DNA content, kinetic complexity, and the ploidy question in Candida albicans

Academic Article

Abstract

  • C. albicans is a dimorphic fungus that is pathogenic for humans. No sexual cycle has been reported for this fungus, and earlier reports have differed on whether typical strains of C. albicans are haploid or diploid. Previous estimates of the DNA content of C. albicans varied by one order of magnitude. The authors used three independent methods to measure the kinetic complexity of the single-copy DNA from a typical strain of C. albicans (strain H317 to determine the DNA content per haploid genote; they obtained values of 15 and 20 fg per cell by using S1 nuclease and hydroxyapatite assays, respectively. Optical assays for DNA reassociation kinetics, although not definitive in themselves, yielded values in this range. Chemical measurements of the DNA content of several typical strains, including strain H317, yielded values clustered about a mean of 37 fg per cell. It is concluded that these strains are diploid.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 22241528
  • Author List

  • Riggsby WS; Torres-Bauza LJ; Wills JW; Townes TM
  • Start Page

  • 853
  • End Page

  • 862
  • Volume

  • 2
  • Issue

  • 7