Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning

Academic Article

Abstract

  • Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. α, β-, and γ-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 12224663
  • Author List

  • Van Tine BA; Dao LD; Wu SY; Sonbuchner TM; Lin BY; Zou N; Chiang CM; Broker TR; Chow LT
  • Start Page

  • 4030
  • End Page

  • 4035
  • Volume

  • 101
  • Issue

  • 12