Estrogen pretreatment protects males against hypoxia-induced immune depression.

Academic Article


  • Hypoxemia depresses cell-mediated immune functions in males, whereas proestrous females do not show such a depression. We hypothesized that elevated systemic estradiol levels in proestrous females prevent hypoxemia-induced immune depression. To study this hypothesis, male C3H/HeN mice were pretreated with 17 beta-estradiol (E(2), 40 microg/kg body wt sc) or vehicle for 3 days before induction of hypoxemia and again immediately before induction of hypoxia. The mice were subjected to hypoxemia (95% N(2)-5% O(2)) or sham hypoxemia (room air) for 60 min, and plasma and spleen cells were collected 2 h later. In vehicle-treated mice, splenocyte proliferation and interleukin-2 and interleukin-3 production were depressed after hypoxemia. E(2)-pretreated animals, however, displayed no such depression in splenic T cell parameters after hypoxemia. Splenic macrophage cytokine production was also depressed in vehicle-treated mice subjected to hypoxia, whereas it was normal in E(2)-pretreated mice. In summary, these findings indicate that administration of E(2) before hypoxemia prevented the depression of cell-mediated immune functions. Thus administration of 17 beta-estradiol in high-risk patients before major surgery might decrease hypoxemia-induced immune depression under those conditions.
  • Keywords

  • Animals, Cells, Cultured, Concanavalin A, Dinoprostone, Estradiol, Female, Hypoxia, Immune System, Interleukins, Lipopolysaccharides, Male, Mice, Mice, Inbred C3H, Random Allocation, Spleen
  • Digital Object Identifier (doi)

    Author List

  • Knöferl MW; Schwacha MG; Jarrar D; Angele MK; Fragoza K; Bland KI; Chaudry IH
  • Start Page

  • C1087
  • End Page

  • C1092
  • Volume

  • 282
  • Issue

  • 5