Biomarkers in Diagnosis of pancreatic carcinoma in fine-needle aspirates.

Academic Article


  • This study was undertaken to determine whether recently identified proteins could be translated to clinical practice as markers to distinguish pancreatic adenocarcinoma from chronic pancreatitis on fine-needle aspirate (FNA) samples. Resected pancreatic tissue sections (n = 40) and FNA samples (n = 65) were stained for clusterin-beta, MUC4, survivin, and mesothelin. For each biomarker, the staining patterns in adenocarcinoma and in reactive ductal epithelium were evaluated and compared. Clusterin-beta stained reactive ductal epithelium significantly more frequently than pancreatic adenocarcinoma (P < .001). In comparison, MUC4 and mesothelin were expressed more frequently in pancreatic adenocarcinoma on tissue sections. Positive staining for MUC4 (91% vs 0%; P < .001) and mesothelin (62% vs 0%; P = .01) and absence of staining for clusterin-beta (90% vs 7%; P < .001) were noted significantly more frequently in adenocarcinoma cells than in reactive cells in FNA samples. Clusterin-beta and MUC4 can help distinguish reactive ductal epithelial cells from the cells of pancreatic adenocarcinoma in FNA samples.
  • Keywords

  • Adenocarcinoma, Biomarkers, Tumor, Biopsy, Fine-Needle, Clusterin, Diagnosis, Differential, Epithelial Cells, GPI-Linked Proteins, Humans, Inhibitor of Apoptosis Proteins, Membrane Glycoproteins, Mesothelin, Microtubule-Associated Proteins, Mucin-4, Mucins, Neoplasm Proteins, Pancreatic Ducts, Pancreatic Neoplasms, Pancreatitis, Research Design, Survivin
  • Digital Object Identifier (doi)

    Pubmed Id

  • 22307581
  • Author List

  • Jhala N; Jhala D; Vickers SM; Eltoum I; Batra SK; Manne U; Eloubeidi M; Jones JJ; Grizzle WE
  • Start Page

  • 572
  • End Page

  • 579
  • Volume

  • 126
  • Issue

  • 4