Phosphoglyceride biosynthesis in bovine adrenal chromaffin cells

Academic Article


  • Cultured adrenal chromaffin cells, representing a virtually homogeneous population of neuronai elements, have been utilized to examine the final enzymes in the formation of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), namely, choline phosphotransferase, ethanolaminephosphotransferase, and the N-methyltransferases in the sequential methylation of PE to PC. Each enzyme has been characterized extensively in terms of substrate requirements, pH optima, detergent and cation effects, and response to inhibitors revealing properties very similar to those in other neural preparations. The respective activities are stable for up to two weeks of adrenal chromaffin cell culture suggesting that this system is a suitable model for examining the relative roles and the regulation of each pathway in PC formation. © 1991 Plenum Publishing Corporation.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Percy AK; Moore JF; Plishker GA; Waymire JC
  • Start Page

  • 505
  • End Page

  • 511
  • Volume

  • 16
  • Issue

  • 4