Structural and immunological characterization of human cytomegalovirus gp55-116 (gB) expressed in insect cells

Academic Article

Abstract

  • The gene encoding the major envelope glycoprotein complex, gp55-116 (gB), of human cytomegalovirus (HCMV) was expressed at high levels in insect cells utilizing a recombinant baculovirus. The mature intracellular form of the insect-derived gp55-116 was a protein of M(r) 150K which contained approximately 50K of N-linked oligosaccharides. The oligosaccharide linkages were almost exclusively endoglycosidase H-sensitive. the 150K protein was processed, presumably by proteolytic cleavage, to yield at least one of the previously defined cleavage products of the gp55-116. this processing step was significantly less efficent in insect cells than the analogous step in mammalian cells. Finally, the insect-derived gp55-116 was highly immunogenic in experimental animals and readily recognized by antibodies contained within HCMV-immune human serum, suggesting that this recombinant protein warrants further study as a potential HCMV subunit vaccine candidate.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Wells DE; Vugler LG; Britt WJ
  • Start Page

  • 873
  • End Page

  • 880
  • Volume

  • 71
  • Issue

  • 4