Stable model immune complexes produced by bivalent affinity labeling haptens: In-vivo survival

Academic Article


  • In order to provide a better model system for studying the properties of immune complexes, we have synthesized a family of bivalent affinity labeling haptens, the p-nitrophenyl (PNP)* and 2,4-dinitrophenyl (DNP) esters of dicarboxylic acids. At low protein concentration, these compounds covalently cross-link anti-PNP and anti-DNP antibodies with considerable specificity. The resulting immunoglobulin oligomers can be separated into stable dimers, trimers, and a mixture of heavier oligomers. The in-vivo clearance in rabbits of these oligomers and of oligomers produced by another bivalent affinity labeling hapten [(BADL-pro)2-EDA] and by a non-specific reagent, dimethylsuberimidate, have been studied. The clearance increased with increasing oligomer size with the dicarboxylic ester-linked immunoglobulin. The in vivo behavior of the other oligomers was irregular. These easily synthesized reagents provide a useful model system for studying some biological properties of immune complexes. © 1979.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Plotz PH; Kimberly RP; Guyer RL; Segal DM
  • Start Page

  • 721
  • End Page

  • 729
  • Volume

  • 16
  • Issue

  • 9