Blocking of targeted microRNAs from next-generation sequencing libraries

Academic Article

Abstract

  • Highly abundant microRNAs (miRNAs) in small RNA sequencing libraries make it difficult to obtain efficient measurements of more lowly expressed species. We present a new method that allows for the selective blocking of specific, abundant miRNAs during preparation of sequencing libraries. This technique is specific with little off-target effects and has no impact on the reproducibility of the measurement of non-targeted species. In human plasma samples, we demonstrate that blocking of highly abundant hsa-miR-16-5p leads to improved detection of lowly expressed miRNAs and more precise measurement of differential expression overall. Furthermore, we establish the ability to target a second abundant miRNA and to multiplex the blocking of two miRNAs simultaneously. For small RNA sequencing, this technique could fill a similar role as do ribosomal or globin removal technologies in messenger RNA sequencing.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Roberts BS; Hardigan AA; Kirby MK; Fitz-Gerald MB; Wilcox CM; Kimberly RP; Myers RM
  • Volume

  • 43
  • Issue

  • 21