Immunoaffinity-purified opiate receptor specifically binds the δ-class opiate receptor ligand, cis-(+)-3-methylfentanylisothiocyanate, SUPERFIT

Academic Article

Abstract

  • Using an antibody generated against the opiate receptor on NG108-15 cells, we recently purified the putative receptor from this hybrid cell line. We herein report that the purified receptor complex specifically binds tritiated cis-(+)-3-methylfentanylisothiocyanate (SUPERFIT), with the predominant binding associated with a 58 kDa polypeptide chain. Consistent with these findings is the in situ labeling of a 58 kDa protein with [3H]SUPERFIT on NG108-15 cells. © 1987.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Carr DJJ; DeCosta B; Jacobson AE; Bost KL; Rice KC; Blalock JE
  • Start Page

  • 272
  • End Page

  • 276
  • Volume

  • 224
  • Issue

  • 2