Extensive variation of human immunodeficiency virus type-1 in vivo

Academic Article


  • Genotypic variation among independent isolates of human immunodeficiency virus type-1 (HIV-1) is well known1-9, but its molecular basis and biological consequences are poorly understood. We examined the genesis of molecular variation in HIV-1 by sequential virus isolations from two chronically infected individuals and analysis of recombinant HIV-1 genomic clones. In three different virus isolates full-length HIV-1 clones were identified and found to consist, respectively, of 17, 9 and 13 distinguishable, but highly-related, viral genotypes. Thirty-five viral clones derived from two HIV-1 isolates obtained from the same individual but 16 months apart showed progressive change, yet were clearly related. Similar changes in the HIV-1 genome did not occur in vitro during virus isolation and amplification. The results indicate that HIV-1 variation in vivo is rapid, that a remarkably large number of related but distinguishable genotypic variants evolve in parallel and coexist during chronic infection, and that 'isolates' of HIV-1, unless molecularly or biologically cloned, generally consist of complex mixtures of genotypically distinguishable viruses. © 1988 Nature Publishing Group.
  • Published In

  • Nature  Journal
  • Digital Object Identifier (doi)

    Author List

  • Saag MS; Hahn BH; Gibbons J; Li Y; Parks ES; Parks WP; Shaw GM
  • Start Page

  • 440
  • End Page

  • 444
  • Volume

  • 334
  • Issue

  • 6181