We evaluated herpes simplex virus type 2 (HSV-2) sero- positivity in an HIV clinic- based population with CD4 lymphocytes counts ≥250 cells/μL and no previous knowledge of HSV-2 infection by history of serology. We demonstrate that although the seropreva- lence of HSV-2 is higher in this HIV-infected population, predictors of HSV-2 seropositivity are similar to those in the general population. Copyright © 2012 American Sexually Transmitted Diseases Association All rights reserved.