Synthetic amphipathic peptides resembling apolipoproteins stimulate the release of human placental lactogen

Academic Article


  • Previous studies from our laboratory demonstrated native high density lipoproteins and apolipoproteins AI, AII, and CI, stimulate the release of human placental lactogen (hPL) from trophoblast cells in culture. To examine the mechanism by which these apolipoproteins stimulate hPL release, we have studied hPL secretion in response to several synthetic peptide analogs of the amphipathic helical structure of the apolipoproteins. The magnitude of the stimulation of hPL release in response to the analog peptides correlated with the ability to displace apolipoproteins from high density lipoprotein and with other measures of phospholipid binding affinity such as the increase of α-helicity and the size of complexes formed between the peptide and phospholipid. The correlation of stimulatory ability and lipid affinity suggests that the action of the apolipoproteins on hPL release may be mediated through an interaction with plasma membrane phospholipids.
  • Published In

    Author List

  • Jorgensen EV; Anantharamaiah GM; Segrest JP; Gwynne JT; Handwerger S
  • Start Page

  • 9215
  • End Page

  • 9219
  • Volume

  • 264
  • Issue

  • 16