Quantitative analysis of total plasma homocysteine by LC-MS/MS

Academic Article


  • Homocysteine is a nonessential, sulfur-containing amino acid involved in onecarbon (folate) metabolism. A number of inherited and acquired conditions cause increased accumulation of this metabolite in blood (homocysteinemia) and other biofluids. Homocysteinemia is a risk factor for cardiovascular disease, including recurrent thrombosis. Accurate measurement of total plasma homocysteine is an important element in the diagnostic evaluation of these disorders. While a number of different methods have been developed for this purpose, the focus of this unit will be on a specific technique utilizing liquid chromatography-tandem mass spectrometry, which provides several advantages in terms of speed, sensitivity, and specificity.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Yuan L; Daniel Sharer J
  • Start Page

  • 17.21.1
  • End Page

  • 17.21.10
  • Volume

  • 2016