Selective downregulation of ANP-clearance-receptor gene expression in lung of rats adapted to hypoxia.

Academic Article

Abstract

  • To test the hypothesis that expression of atrial natriuretic peptide (ANP)-receptor genes is modified to provide a compensatory mechanism against hypoxic pulmonary hypertension, steady state mRNA levels for the ANP-A receptor (or guanylate cyclase-A; ANPAR), ANP-B receptor (or guanylate cyclase-B; ANPBR), and ANP-clearance receptor (ANPCR) were quantitated by Northern blot and slot-blot analysis in lung, kidney, spleen, and liver of hypoxia-adapted rats and air controls. Exposure of rats to short-term (48 h) and chronic (4 wk) hypoxia (10% O2, 1 atm) did not affect lung ANPAR-mRNA levels. Lung ANPBR-mRNA levels were unchanged by short-term hypoxia but selectively increased (approximately twofold) by chronic hypoxia. ANPCR-mRNA levels were selectively and significantly downregulated by 48-h and 4-wk hypoxia in lung but were unchanged or upregulated in other tissues. Lung ANPCR gene transcription, assessed by nuclear-runoff analysis, was decreased by hypoxia. These data support the conclusion that altered pulmonary ANP-receptor gene expression modulates the development of hypoxic pulmonary hypertension.
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    Published In

    Keywords

  • Adaptation, Physiological, Animals, Base Sequence, Gene Expression, Hypoxia, Kidney, Liver, Lung, Male, Molecular Probes, Molecular Sequence Data, RNA, Messenger, Rats, Rats, Sprague-Dawley, Receptors, Atrial Natriuretic Factor, Spleen
  • Digital Object Identifier (doi)

    Author List

  • Li H; Oparil S; Meng QC; Elton TS; Chen YF
  • Start Page

  • L328
  • End Page

  • L335
  • Volume

  • 268
  • Issue

  • 2 Pt 1