A functional comparison of the rat type-1 angiotensin II receptors (AT1AR and AT1BR)

Academic Article

Abstract

  • To evaluate and functionally compare the rat AT and AT receptor subtypes, stable Chinese hamster ovary (CHO) cell lines expressing either recombinant receptor in approximately equal numbers were generated. Radioligand binding data suggests that the recombinant AT receptor is pharmacologically similar to the recombinant AT receptor. Functional studies indicate that both receptor subtypes can independently activate the phospholipase C IP and the dihydropyridine-sensitive voltage-dependent Ca channel signal transduction pathways with equal efficiency, but are unable to modulate cAMP accumulation under our experimental conditions. Furthermore, both receptors can be directly involved in the cellular growth properties of AII. Slot-blot experiments clearly demonstrate that these receptors are expressed in a tissue-specific manner. A sequence comparison of the 5′ flanking regions of these two genes shows that they have very little sequence homology (∼ 36%), suggesting that although the AT and AT receptors appear to be pharmacologically and functionally similar, the control of their expression seems to be governed by distinct transcription factors. © 1995. 1A 1B 1A 1B 3 1A 1B 2+
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Martin MM; White CR; Li H; Miller PJ; Elton TS
  • Start Page

  • 135
  • End Page

  • 147
  • Volume

  • 60
  • Issue

  • 2-3