Strategies for the rapid construction of conditionally-replicating HSV-1 vectors expressing foreign genes as anticancer therapeutic agents

Academic Article

Abstract

  • Conditionally replication-competent Herpes Simplex Virus Type 1 (HSV-1) vectors expressing foreign genes have been developed as experimental therapeutic agents. Traditional methods of virus construction, including growth selection based on thymidine kinase gene expression, and color selection based on a reporter gene expression are often time-consuming and relatively inefficient. This review summarizes the various strategies developed in recent years for the rapid and efficient construction of novel conditionally replication-competent mutant HSV expressing multiple foreign genes. Additionally, two new modifications of existing strategies, which have not been previously reported, are discussed. © 2011 American Chemical Society.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Parker JN; Zheng X; Luckett W; Markert JM; Cassady KA
  • Start Page

  • 44
  • End Page

  • 49
  • Volume

  • 8
  • Issue

  • 1