Isolation of T-lymphocytes from disaggregated tumors, with high purity and good percentage recovery: brief communication

Academic Article

Abstract

  • A combination of 2 cell separation methods was utilized for the isolation of thymus derived lymphocytes (TL) from enzymatically disaggregated tumors. Passage through Sephadex G 10 glass bead columns to remove adherent cell types followed by exposure to IgG coated sheep red blood cell monolayers for removal of Fc receptor bearing inflammatory cells provided functional TL suspensions of high purity with good percentage recovery.
  • Digital Object Identifier (doi)

    Author List

  • Hansen CB; Gillespie GY; Russell SW
  • Start Page

  • 273
  • End Page

  • 275
  • Volume

  • 59
  • Issue

  • 1