The remodelled tracheal basement membrane zone of infant rhesus monkeys after 6 months of recovery

Academic Article

Abstract

  • Background: In previous studies, we showed that repeated exposure to (1) house dust mite allergen (HDMA) (Dermatophagoides farinae) caused thickening of the basement membrane zone (BMZ) and (2) HDMA+ozone (O3) caused depletion of BMZ perlecan and atypical development of BMZ collagen (irregular thin areas < 2.0 μm in width). Objective: The purpose of this study was to determine if these remodelling changes were reversible after 6 months of recovery. Methods: Rhesus monkeys were exposed to a regimen of HDMA and or O3 or filtered air (FA) for 6 months. After the exposure protocol was completed FA and O3 groups were allowed to recover in FA for 6 months. The HDMA and HDMA+O3 exposure groups recovered in a modified environment. They were re-exposed to HDMA aerosol for 2 h at monthly intervals during recovery in order to maintain sensitization for pulmonary function testing. To detect structural changes in the BMZ, collagen I and perlecan immunoreactivity were measured and compared to data from the previous papers. Results: The remodelled HDMA group had a significantly thicker BMZ and after 6 months of recovery the width had not regressed. In the remodelled BMZ of the HDMA+O3 group, perlecan had returned to the BMZ after 6 months of the recovery protocol, and the thin, irregular, collagen BMZ had been resolved. Conclusion: In summary, this study has shown that: (1) The width of the remodelled HDMA BMZ did not regress during a recovery protocol that included a sensitizing dose of HDMA. (2) The atypical collagen BMZ in the HDMA+O 3 BMZ was resolved in the absence of O3. (3) Depletion of perlecan from the BMZ by O3 was reversed by recovery in the absence of O3.
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    Digital Object Identifier (doi)

    Author List

  • Evans MJ; Fanucchi MV; Baker GL; Van Winkle LS; Pantle LM; Nishio SJ; Schelegle ES; Gershwin LJ; Miller LA; Hyde DM
  • Start Page

  • 1131
  • End Page

  • 1136
  • Volume

  • 34
  • Issue

  • 7