Multi-point estimation of total energy expenditure: A comparison between zinc-reduction and platinum-equilibration methodologies

Academic Article


  • Reducing water to hydrogen gas by zinc or uranium metal for determining D/ H ratio is both tedious and time consuming. This has forced most energy metabolism investigators to use the "two-point" technique instead of the "Multi-point" technique for estimating total energy expenditure (TEE). Recently, we purchased a new platinum (Pt)-equilibration system that significantly reduces both time and labor required for D/H ratio determination. In this study, we compared TEE obtained from nine overweight but healthy subjects, estimated using the traditional Zn-reduction method to that obtained from the new Pt-equilibration system. Rate constants, pool spaces, and CO 2 production rates obtained from use of the two methodologies were not significantly different. Correlation analysis demonstrated that TEEs estimated using the two methods were significantly correlated (r=0.925, p=0.0001). Sample equilibration time was reduced by 66% compared to those of similar methods. The data demonstrated that the Zn-reduction method could be replaced by the Pt-equilibration method when TEE was estimated using the "Multi-Point" technique. Furthermore, D equilibration time was significantly reduced. © 2003 Elsevier Inc. All rights reserved.
  • Authors

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    Digital Object Identifier (doi)

    Author List

  • Sonko BJ; Miller LV; Jones RH; Donnelly JE; Jacobsen DJ; Hill JO; Fennessey PV
  • Start Page

  • 211
  • End Page

  • 217
  • Volume

  • 323
  • Issue

  • 2