Mutagenesis of T cell antigen receptor ζ chain tyrosine residues. Effects on tyrosine phosphorylation and lymphokine production

Academic Article

Abstract

  • Occupancy of the T cell antigen receptor triggers a complex set of events that culminate in cellular activation. It is clear that tyrosine kinases play important roles in this process. The ζ subunit of the T cell antigen receptor is a 16-kDa transmembrane structure that exists primarily as a disulfide-linked homodimer. On receptor activation, a subset of ζ molecules undergo tyrosine phosphorylation. To evaluate this process and the role of ζ phosphorylation in T cell activation, site-specific mutagenesis of the intracytoplasmic tyrosines of ζ has been carried out. Analysis of cells expressing these mutant ζ subunits demonstrated that multiple tyrosines underwent phosphorylation in response to receptor engagement, and that the four most carboxyl tyrosines were most crucial to this process. Despite abnormalities in phosphorylation induced by the mutations, lymphokine production in these transfectants was unaffected. Hence, although ζ is a prominent substrate for a receptor-activated tyrosine kinase, neither the mutation of individual tyrosines nor the alteration of the phosphorylation state of the molecule substantively affected the coupling of T cell receptor activation to lymphokine production. These findings raise questions regarding the role of ζ phosphorylation in T cell activation.
  • Published In

    Author List

  • Frank SJ; Cenciarelli C; Niklinska BB; Letourneur F; Ashwell JD; Weissman AM
  • Start Page

  • 13656
  • End Page

  • 13660
  • Volume

  • 267
  • Issue

  • 19