Inhibition of xanthine oxidase by uric acid and its influence on superoxide radical production

Academic Article

Abstract

  • The inhibition of xanthine oxidase by its reaction product, uric acid, was studied by steady state kinetic analysis. Uric acid behaved as an uncompetitive inhibitor of xanthine oxidase with respect to the reducing substrate, xanthine. Under 50 μM xanthine and 210 μM oxygen, the apparent Ki for uric acid was 70 μM. Uric acid-mediated xanthine oxidase inhibition also caused an increase in the percentage of univalent reoxidation of the enzyme (superoxide radical production). Steady-state rate equations derived by the King-Altman method support the formation of an abortive-inhibitory enzyme-uric acid complex (dead-end product inhibition). Alternatively, inhibition could also depend on the reversibility of the classical ping-pong mechanism present in xanthine oxidase-catalyzed reactions. © 1992.
  • Authors

    Digital Object Identifier (doi)

    Pubmed Id

  • 11606100
  • Author List

  • Radi R; Tan S; Prodanov E; Evans RA; Parks DA
  • Start Page

  • 178
  • End Page

  • 182
  • Volume

  • 1122
  • Issue

  • 2