Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina

Academic Article


  • This study is to test anatomic correlates, including connecting cilium (CC) and inner segment (IS) ellipsoid, to the hyper-reflective band visualized by optical coherence tomography (OCT) and commonly attributed to the photore-ceptor inner/outer segment (IS/OS) junction.Aline-scan OCT (LS-OCT) was constructed to achieve sub-cellular resolution (lateral: ∼2 μm; axial: ∼4 μm) of excised living frog retinas. Anelectro-optic phase modulator was employed for rapid and vibration-free phase modulation. Comparison of normalized distance measurements between LS-OCT images and histological images revealed that the dominant source of the signal reported as the IS/OS OCT band actually originates from the IS. © 2012 Society ofPhoto-Optical Instrumentation Engineers (SPIE).
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Lu RW; Curcio CA; Zhang Y; Zhang QX; Pittler SJ; Deretic D; Yao XC
  • Volume

  • 17
  • Issue

  • 6