CD28 down-regulation on CD4 T cells is a marker for graft dysfunction in lung transplant recipients

Academic Article


  • Rationale: Repeated antigen-driven proliferations cause CD28 on T cells to down-regulate. We hypothesized that alloantigen-induced proliferations could cause CD28 down-regulation in lung transplant recipients. Objectives: To ascertain if CD28 down-regulation on CD4 T cells associated with manifestations of allograft dysfunction in lung transplant recipients. Methods: Peripheral blood CD4 T cells from 65 recipients were analyzed by flow cytometry, cytokine multiplex and proliferative assays, and correlated with clinical events. Measurements and Main Results: Findings that CD28 was present on less than 90% of total CD4 T cells were predominantly seen among the recipients with bronchiolitis obliterans syndrome (specificity = 88%). Perforin and granzyme B were produced by >50% of the CD4 CD28 cells, but less than 6% of autologous CD4 CD28 cells (P < 0.006). CD4 CD28 cells also had increased productions of proinflammatory cytokines, but less frequently expressed regulatory T-cell marker FoxP3 (2.1 ± 1.3%), compared with autologous CD4 CD28 (9.5 ± 1.4; P = 0.01). Cyclosporine A (100 ng/ml) inhibited proliferation of CD4 CD28 cells by 33 ± 11% versus 68 ± 12% inhibition of CD4 CD28 (P = 0.025). FEV fell 6 months later (0.35±0.04L) in recipients with CD4 CD28 /CD4 less than 90% (CD28% Low) compared with 0.08 ± 0.08 L among CD4 CD28 /CD4 (CD28% High) greater than 90% (CD28% High) recipients (P = 0.013). Two-year freedom from death or retransplantation in CD28% Low recipients was 32 ± 10% versus 78 ± 6% among the CD28% High subjects (P < 0.0001). Conclusions: CD28 down-regulation on CD4 cells is associated with bronchiolitis obliterans syndrome and poor outcomes in lung transplantation recipients. CD4 CD28 cells have unusual, potentially pathogenic characteristics, and could be important in the progression of allograft dysfunction. These findings may illuminate a novel paradigm of transplantation immunopathogenesis, and suggest that CD28 measurements could identify recipients at risk for clinical deteriorations. + null + + + null + + + null + + + + + + + null 1 total total
  • Digital Object Identifier (doi)

    Author List

  • Studer SM; George MP; Zhu X; Song Y; Valentine VG; Stoner MW; Sethi J; Steele C; Duncan SR
  • Start Page

  • 765
  • End Page

  • 773
  • Volume

  • 178
  • Issue

  • 7