Cryo-EM of Helical Polymers

Academic Article


  • While the application of cryogenic electron microscopy (cryo-EM) to helical polymers in biology has a long history, due to the huge number of helical macromolecular assemblies in viruses, bacteria, archaea, and eukaryotes, the use of cryo-EM to study synthetic soft matter noncovalent polymers has been much more limited. This has mainly been due to the lack of familiarity with cryo-EM in the materials science and chemistry communities, in contrast to the fact that cryo-EM was developed as a biological technique. Nevertheless, the relatively few structures of self-assembled peptide nanotubes and ribbons solved at near-atomic resolution by cryo-EM have demonstrated that cryo-EM should be the method of choice for a structural analysis of synthetic helical filaments. In addition, cryo-EM has also demonstrated that the self-assembly of soft matter polymers has enormous potential for polymorphism, something that may be obscured by techniques such as scattering and spectroscopy. These cryo-EM structures have revealed how far we currently are from being able to predict the structure of these polymers due to their chaotic self-assembly behavior.
  • Authors

    Published In

  • Chemical Reviews  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 27837050
  • Author List

  • Wang F; Gnewou O; Solemanifar A; Conticello VP; Egelman EH
  • Start Page

  • 14055
  • End Page

  • 14065
  • Volume

  • 122
  • Issue

  • 17